<p class="Default">Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antioksidan dan antidiabetes ekstrak etil asetat daun katemas (Euphorbia heterophylla L.) secara in vitro dan in silico melalui inhibisi terhadap enzim α-glukosidase. Pada penelitian ini ekstraksi sampel dilakukan menggunakan maserasi bertingkat, dimulai dengan n-heksana, selanjutnya dengan etil asetat. Ekstrak etil asetat yang didapatkan dilakukan pengujian kuantitiatif total fenolik dan flavonoid. Uji aktivitas antioksidan terhadap ekstrak dilakukan menggunakan metode DPPH. Uji aktivitas antidiabetes terhadap ekstrak dilakukan secara in vitro dan in silico melalui inhibisi terhadap enzim α-glukosidase menggunakan akarbose sebagai standar. Uji aktivitas antidiabetes terhadap kandungan senyawa bioaktif ekstrak secara in silico atau molecular docking menggunakan software Discovery Studio 4.1. Hasil penelitian menunjukkan bahwa nilai total fenolik dari ekstrak adalah 4,24 mg GAE/g berat kering dan nilai flavonoid total adalah: 3,22 mg KE/g berat kering. Hasil uji aktivitas antioksidan ekstrak didapatkan nilai IC50 sebesar 37,56 µg/mL, digolongkan sebagai aktivitas antioksidan yang sangat kuat. Hasil uji aktivitas antidiabetes secara in vitro didapatkan nilai IC50 sebesar 138,63 µg/mL. Hasil molecular docking memperlihatkan bahwa senyawasenyawa aktif yang terdapat didalam ekstrak mampu membentuk ikatan hidrogen antara ligan dengan reseptor, tapi lebih sedikit jika dibandingkan dengan akarbose.</p><p class="Default"><strong>Measurement of Total Phenolic, Flavonoids, Antioxidant and Antidiabetic Activity of Catemas Leaf Ethyl Acetate Extract (Euphorbia heterophylla L.) by In Vitro and In Silico through Enzim α-Glucosidase Inhibition.</strong> This study aims to determine the antidiabetic activity of katemas (Euphorbia heterophylla L.) ethyl acetate extract in vitro and in silico through inhibition of the αglucosidase enzyme. In this study, the sample extraction was carried out by multilevel maceration, starting with n-hexane, then with ethyl acetate. The ethyl acetate extract obtained was quantitatively tested by total phenolic and flavonoids. The antioxidant activity of the extract was tested using the DPPH method. The antidiabetic activity of the extract was examined through inhibiting the enzyme αglucosidase in vitro using a microplate reader and in silico (molecular docking) using Discovery Studio 4.1 software. The results showed that the total phenolic value of the extract was 4.24 mg GAE/g of dry weight, and the total flavonoid value was 3.22 mg KE/g of dry weight. Antioxidant activity test obtained IC50 of 37,56 µg/mL, classified as verry strong antioxidant. The in vitro antidiabetic test examined that IC50 is 138.63 µg/mL. The results of molecular docking showed that the active compounds in the extracts are able to form hydrogen bonds between ligand and receptor; however, the amount was less than the hydrogen bonds formed by acarbose.</p>

<p class="Default">Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antidiabetes ekstrak etil asetat daun Katemas (<em>Euphorbia heterophylla,</em> L.) secara <em>in vitro</em> dan <em>in silico</em> melalui inhibisi terhadap enzim α-glukosidase.Pada penelitian ini ekstraksi sampel dilakukan menggunakan maserasi bertingkat, dimulai dengan n-heksan, selanjutnya dengan etil asetat.Ekstrak etil asetat yang didapatkan dilakukan pengujiankuantitiatif total fenolik dan flavonoid. Terhadap ekstrak dilakukan uji aktivitas aktivitas antidiabetes melalui inhibisi terhadap enzim α-glukosidasesecara in vitro menggunakan <em>microplate reader</em> dan<em>in silico</em> menggunakan <em>molecular docking</em> menggunakan<em> software </em>Discovery Studio 4.1. Hasil penelitian menunjukkan bahwa nilai total fenolik dari ekstrak adalah 4,24 mg K/g berat keringdan nilai flavonoid total adalah: 3,22 mg K/g berat kering. Hasil uji aktivitas antidiabetes secara <em>in vitro</em> didapatkan nilai <em>IC</em>₅₀ sebesar 138,63 µg/mL. Hasil <em>molecular docking </em>memperlihatkan bahwa senyawa-senyawa aktif yang terdapat didalam ekstrak mampu membentuk ikatan hidrogen antara ligan dengan reseptor, tapi lebih sedikit jika dibandingkan dengan akarbose. </p>

The purpose of this study is to isolate, purify and characterize active compounds from E. heterophylla L. plants and to know the antidiabetic activity of pure Katemas (E.heterophylla, L.) leaf compounds in vitro and in silico (molecular docking) through inhibition of alpha glucosidase enzymes. In this study sample extraction was carried out using multilevel maceration, starting with n-hexane, then with ethyl acetate. Isolation was done by VLC. Antidiabetic activity was tested for pure compounds through inhibition of the αglucosidase enzyme in vitro using a microplate reader and in silico (molecular docking) using Discovery Studio 4.1 software. VLC results from the ethyl acetate extract of katemas leaves obtained 21 fractions and taken f2 because of the potential for more and stable crystals. F2 is predicted to be a DS-01 diterpenoid compound according to the results of UV, IR, H 1-NMR, C 13-NMR and MS spectroscopy. The results of antidiabetic activity test in vitro obtained IC 50 values of 138.63 µg/mL for ethyl acetate extract and IC 50 values of 191.82 µg/mL for diterpenoid DS-01 compounds. The results of molecular docking showed that the active compounds contained in the extract and also DS-01 diterpenoids were able to form hydrogen bonds between the ligand with the receptor, but less when compared with the acarbose.