Testing hypotheses of neuromuscular function during locomotion ideally requires the ability to record cellular responses and to stimulate the cells being investigated to observe downstream behaviors [1]. The inability to stimulate in free flight has been a long-standing hurdle for insect flight studies. The miniaturization of computation and communication technologies has delivered ultra-small, radio-enabled neuromuscular recorders and stimulators for untethered insects [2-8]. Published stimulation targets include the areas in brain potentially responsible for pattern generation in locomotion [5], the nerve chord for abdominal flexion [9], antennal muscles [2, 10], and the flight muscles (or their excitatory junctions) [7, 11-13]. However, neither fine nor graded control of turning has been demonstrated in free flight, and responses to the stimulation vary widely [2, 5, 7, 9]. Technological limitations have precluded hypotheses of function validation requiring exogenous stimulation during flight. We investigated the role of a muscle involved in wing articulation during flight in a coleopteran. We set out to identify muscles whose stimulation produced a graded turning in free flight, a feat that would enable fine steering control not previously demonstrated. We anticipated that gradation might arise either as a function of the phase of muscle firing relative to the wing stroke (as in the classic fly b1 muscle [14, 15] or the dorsal longitudinal and ventral muscles of moth [16]), or due to regulated tonic control, in which phase-independent summation of twitch responses produces varying amounts of force delivered to the wing linkages [15, 17, 18].

In this study, a biological microactuator was demonstrated by closed-loop motion control of the front leg of an insect (Mecynorrhina torquata, beetle) via electrical stimulation of the leg muscles. The three antagonistic pairs of muscle groups in the front leg enabled the actuator to have three degrees of freedom: protraction/retraction, levation/depression, and extension/flexion. We observed that the threshold amplitude (voltage) required to elicit leg motions was approximately 1.0 V; thus, we fixed the stimulation amplitude at 1.5 V to ensure a muscle response. The leg motions were finely graded by alternation of the stimulation frequencies: higher stimulation frequencies elicited larger leg angular displacement. A closed-loop control system was then developed, where the stimulation frequency was the manipulated variable for leg-muscle stimulation (output from the final control element to the leg muscle) and the angular displacement of the leg motion was the system response. This closed-loop control system, with an optimized proportional gain and update time, regulated the leg to set at predetermined angular positions. The average electrical stimulation power consumption per muscle group was 148 µW. These findings related to and demonstrations of the leg motion control offer promise for the future development of a reliable, low-power, biological legged machine (i.e., an insect–machine hybrid legged robot).

This paper addresses the problem of detecting the presence of malware that leave periodic traces in network traffic. This characteristic behavior of malware was found to be surprisingly prevalent in a parallel study. To this end, we propose a visual analytics solution that supports both automatic detection and manual inspection of periodic signals hidden in network traffic. The detected periodic signals are visually verified in an overview using a circular graph and two stacked histograms as well as in detail using deep packet inspection. Our approach offers the capability to detect complex periodic patterns, but avoids the unverifiability issue often encountered in related work. The periodicity assumption imposed on malware behavior is a relatively weak assumption, but initial evaluations with a simulated scenario as well as a publicly available network capture demonstrate its applicability

been tested to extend the shelf life of banana storage. Cold plasma is an environmentally friendly antimicrobial technology with the potential to enhance the shelf life of fresh fruit and vegetables. nonequilibrium atmospheric pressure microsecond-pulsed direct barrier discharge plasma (msp-DBD or "cold plasma") on the shelf life of bananas. Bananas were exposed to plasma-treated air for 1 week at room temperature and normal pressure and humidity. The change in weight, color, surface morphology, and sugar content of bananas was investigated. Plasma treatment caused no ally, mold growth was observed in untreated samples after storage, but was absent in plasmatreated samples. This study demonstrated that the mspDBD technique has the potential to prolong the shelf life of bananas compared with conventional methods by inhibiting pathogen growth in post-harvest storage conditions.