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Silver nanoparticles (AgNPs) were prepared by DC high voltage electrochemical method using a silver anode. The results showed that, for all prepared samples, depending on the size and shape of their nanoparticles, a well‐defined UV‐Vis peak in aqueous solution induced by the surface Plasmon property of AgNPs was observed at wavelength values in the range of 400‐450 nm. Based on the linear dependence between AgNPs concentration and UV‐Vis absorbance up to 200 ppm with the R value of 0.9989, the concentration of AgNPs can be derived quantitatively. Thus, this study introduced a simple quantitative method to determine the concentration of silver nanoparticles directly, instead of the total ionic silver that other chemical, biological or physical methods usually provided.

Nano silver was known as a bactericidal agent for almost all bacterial species and has been intensively investigated over the world to apply for disinfection in different fields of human activities and environmental protection. Specifically, genital exudate media contain numerous dangerous bacteria such as Neisseria gonorrhoeae, Enterococcus faecalis, Staphylococcus aureus, Candida albicans etc. which as a rule are drug-resistant with respect to antibiotics. This research presents the results of the application of nanosilver in replacing antibiotics in elimination of the above mentioned microorganisms by using low nanosilver concentrations of about 0.01 mg/cm2 immobilized on non-woven fabric. The experimental data showed that nanosilver-impregnated fabric samples at a concentration 0.01 mg/cm2 gave antibacterial rings of 10 mm, 9 mm and 8 mm, respectively, for        N. gonorrhoeae, Candida albicans + E.coli and Candida albicans + E.coli + S. aureus.

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