There have been few reports on the imaging characteristics of cryptococcus neoformans (C. neoformans ) infection of the breast. Herein, we reported the imaging features of C. neoformans infection of the breast in a 41-year-old woman with immune thrombocytopenic purpura. Bilateral, diffuse, hyperechoic, and well-defined margin lesions were observed on breast ultrasounds. In addition, a global asymmetry in the left breast, and a focal asymmetry in the right breast were observed on mammograms. Breast fine needle aspiration and biopsy results revealed a C. neoformans infection. After 5 months of treatment with oral fluconazole and amphotericin B, the lesion on the right breast disappeared on repeated-breast ultrasounds.

Rice bran is an important source of nutrients that have many good bioactive compounds. This study examined the extraction of bran rice oil using supercritical carbon dioxide. Free fatty acids contained in bran rice were stabilized at 5.25% for 8 months by fluid bed dryer equipment. Supercritical carbon dioxide extraction of rice bran oil at pressure of 400 bar, temperature of 60 oC, CO2 flow rate of 20 g/min for 120 minutes yielded 14.84% oil. The concentration of γ-oryzanol in rice bran oil extracted by supercritical carbon dioxide (0.50%) was higher than in rice bran oil derived from hexane Soxhlet extraction (0.42%). The effect of pressure and temperature on extraction yield and the concentration of γ-oryzanol contained in rice bran oil was observed. Keywords Rice bran, rice bran oil, γ-oryzanol, free fatty acid, supercritical carbon dioxide. References [1] A. A. Wani, P. Singh, M. A. Shah, U. S. Weisz, K. Gul, I. A. Wani, Rice Starch Diversity: Effects on Structural, Morphological, Thermal, and Physicochemical Properties -A Review, Comprehensive Reviews in Food Science and Food Safety, Vol. 11, No. 5, 2012, pp. 417-436, https://doi.org/10.1111/j.1541-4337.2012.00193.x.[2] K. Gul, B. Yousuf, A. K. Singh, P. Singh, A. A. Wani, Rice Bran: Nutritional Values and Its Emerging Potential for Development of Functional Food - A Review, Bioactive Carbohydrates and Dietary Fibre, Vol. 6, No. 1, 2015, pp. 24-30, https://doi.org/10.1016/j.bcdf.2015.06.002.[3] G. Hua, S. Huang, S. Cao, Z. Ma, Effect of Enrichment with Hemicellulose from Rice Bran on Chemical and Functional Properties of Bread, Food Chemistry, Vol. 115, No. 3, 2009, pp. 839-842, https://doi.org/10.1016/j.foodchem.2008.12.092.[4] T. S. Shin, J. S. Godber, Changes of Endogenous Antioxidants and Fatty Acid Composition in Irradiated Rice Bran during Storage, Journal of Agricultural Food Chemistry, Vol. 44, 1996, pp. 567-573, https://doi.org/10.1021/jf950386a.[5] B. O. Juliano, P. A. Hicks, Rice Functional Properties and Rice Food Products, Food Reviews International, Vol. 12, No. 1, 1996, pp. 71-103, https://doi.org/10.1080/87559129609541068.[6] M. Ghosh, Review on Recent Trends in Rice Bran Oil Processing, J Amer Oil Chem Soc,Vol. 84, 2007, pp. 315-324, https://doi.org/10.1007/s11746-007-1047-3.[7] M. Sugano, E. Tsuji, Rice Bran Oil and Cholesterol Metabolism, Journal of Nutrition, Vol. 127, No. 3, 1997, pp. 521-524, https://doi.org/10.1093/jn/127.3.521S.[8] G. S. Seetharamaiah, N. Chandrasekhara, Studies on Hypocholesterolemic Activity of Rice Bran Oil, Atherosclerosis, Vol. 78, No. 2-3, 1989, pp. 219-223, https://doi.org/10.1016/0021-9150(89)90226-8.[9] J. K. Duve, P. J. White, Extraction and Identification of Antioxidants in Oats, Journal of American Oil Chemists Society, Vol. 68, No. 6, 1991, pp. 365-37, https://doi.org/10.1007/BF02663751.[10] L. Xu, X. Zhan, Z. Zeng, R. Chen, H. Li, T. Xie, S. Wang, Recent Advances on Supercritical Fluid Extraction of Essential Oils, African Journal of Pharmacy and Pharmacology, Vol. 5, No. 9, 2011, pp. 1196-1211, https://doi.org/10.5897/AJPP11.228.[11] C. R. Chen, C. H. Wang, L. Y. Wang, Z. H. Hong, S. H. Chen, W. J. Ho, C. M. J. Chang, Supercritical Carbon Dioxide Extraction and Deacidification of Rice Bran Oil, J. of Supercritical Fluids, Vol. 45, No. 3, 2008, pp. 322-331, https://doi.org/10.1016/j.supflu.2008.01.006.[12] K. Tomita, S. Machmudah, Wahyudiono, R. Fukuzato, H. Kanda, A. T. Quitain, M. Sasaki, M. Goto, Extraction of Rice Bran Oil by Supercritical Carbon Dioxide and Solubility Consideration, Separation and Purification Technology, Vol. 125, No. 7, 2014, pp. 319-325, http://dx.doi.org/10.1016/j.seppur.2014.02.008.[13] F. Malekian, R. M. Rao, W. Prinyawiwatkul, W. E. Marshall, M. Windhauser, M. Ahmedna, Lipase and Lipoxygenase Activity, Functionality, and Nutrient Losses in Rice Bran During Storage, Bull. La. Agric. Exp. Stn. LSU Agric. Cent., Vol. 870, 2000, pp. 1-68.[14] S. Akter, M. Ahiduzzaman, Effect of Storage Life of Rice Bran on the Quality of Oil, Journal of Food and Nutrition Sciences, Vol. 5, No. 1, 2017, pp. 11-15, https://doi.org/10.11648/j.jfns.20170501.12.[15] H. R. Sharma, G. S. Chauhan, K. Agrawal, Physico-Chemical Characteristics of Rice Bran Processed by Dry Heating and Extrusion Cooking, International Journal of Food Properties, Vol. 7, No. 3, 2004, pp. 603-614, https://doi.org/10.1081/JFP-200033047.[16] N. R. Lakkakula, M. Lima, T. Walker, Rice Bran Stabilization and Rice Bran Oil Extraction using Ohmic Heating, Bioresource Technology, Vol. 92, No. 2, 2004, pp. 157-161, https://doi.org/10.1016/j.biortech.2003.08.010.[17] D. Martin, J. S. Gober, G. Selhako, L. Verma, J. H. Wells, Optimizing Rice Bran Stabilization by Extrusion Cooking, Lousiana Agraculture, Vol. 30, 1993, pp. 13.[18] R. N. Sayre, R. M. Saunders, R. V. Enochian, W. G. Shultz, E. C. Beagle, Review of Rice Bran Stabilization Systems with Emphasis on Extrusion Cooking, Cereal Food World, Vol. 7, 1982, pp. 317.[19] M. S. Kuk, M. K. Dowd, Supercritical CO2 Extraction of Rice Bran, J. Am. Oil Chem. Soc, Vol. 75, 1998, pp. 623-628.[20] Y. T. Chen, Y. H. Ling, An Overview of Supercritical Fluid Extraction in Chinese Herbal Medicine: from Preparation to Analysis, J. Food Drug Anal, Vol. 8, No. 4, 2000, pp. 235-247, https://doi.org/10.38212/2224-6614.2815.[21] S. R. S. Ferreira, Z. L. Nikolov, L. K. Doraiswamy, M. A. A. Meireles, A. J. Petenatee, Supercritical Fluid Extraction of Black Pepper (Piper nigrun L.) Essential Oil, J. Supercrit. Fluids, Vol. 14, No. 3, 1999, pp. 235-245.[22] A. Awasthi, R. K. Trivedi, A Review of Supercritical Carbon Dioxide Extraction of Natural Products, Chem. Eng. World, Vol. 32, 1997, pp. 65-71.    

Isoflavones are secondary phenolic metabolites found in most legumes. These compounds have important pharmacological significance such as anti-osteoporosis, anti-aging, and anti-cancer properties. Breast cancer is one of the most common cancers in women worldwide. Human epidermal growth factor receptor-2 (HER2) is an important target in breast cancer treatment. In this study, we evaluated the ability of sixty isoflavones compounds to inhibit the HER2 enzyme for their potential use in breast cancer treatments by the molecular docking method. Molecular docking was done by Autodock vina software. Lipinski 5 rule is used to compare compounds with drug-like and non-drug-like properties. Pharmacokinetic parameters of potential compounds were evaluated using the pkCSM tool. Our results showed that 35 compounds inhibited HER2 stronger than the positive control. Next, we analysed the drug-likeness according to Lipinski’s rule of five and predicted pharmacokinetic-toxicological parameters of these 35 compounds. We obtained two compounds, genistein and biochanin A, which could become promising drugs for breast cancer treatment. However, in vitro and in vivo studies on the inhibition of the HER2 enzyme need to be conducted.

Hemorrhagic stroke due to ruptured brain arteriovenous malformations (AVMs) is a common cause in young stroke patients. When the ruptured AVMs are in deep location, the choice of endovascular intervention with the arterial approach to AVM embolization is routine but in many cases, it is not feasible due to the inability to access because of the small and tortuous arterial branch, however, the intravenous approach also results in high complete obliteration rates but also carries a higher risk of stroke than the intra-arterial route. We describe a 36-year-old female patient diagnosed with intracranial and intraventricular hemorrhage who underwent complete transvenous embolization of the ruptured AVMs, and achieved near-complete clinical recovery after 1 month with the modified Rankin scale 1.

Triterpenoid is one of the compounds that contribute tored lingzhi mushroom's major significant therapeutic activities (G. lucidum). Triterpenoids have anti-allergic, anti-cancer, anti-tumor, and anti-aging effects, enhance oxygen utilization, free radical scavenging, lowerblood pressure, inhibit cholesterol biosynthesis, and enhance liver function and blood pressure, inhibit cholesterol biosynthesis, and improve liver functionetc. The beneficial effects of triterpenoids in Ganoderma lucidum on human health have generated interest in developing methods for extracting them from the fruit bodies of G. lucidum. This article presents the effect of solvent in combination with ultrasonic wave assistance in triterpenoid extraction ability from red lingzhi mushroom. Before carrying out triterpenoid extraction; Red lingzhi mushroom was ground 2mm in size. Four parameters of the extraction process, including the ethanol concentration, ratio (v:w) between ethanol solvent and mushroom, extraction time, , extraction temperature, ultrasonic time and ultrasonic intensity were determined. The extraction without ultrasonic waves for 180 minutes was used as a control. The total triterpenoid content was collected during the extraction process. The results showed that red lingzhi mushroom was extracted by ethanol solvent at a concentration of 85%, the ratio between ethanol solvent and mushroom was 3:1 (v:w), ultrasound intensity was 58 W/cm2, frequency was 20 kHz), extracting temperature was 55°C, removing time was 5 min had total triterpenoid content 3.06 times higher than the control.