The research objective was to analyze the effect of role conflict on employee job satisfaction through work stress mediation. This causality-associative research conducted at the Gianyar Regency Land Office involved 87 respondents with the sampling method using saturated sampling techniques. Data were collected by means of a questionnaire, and analyzed using descriptive analysis, analysis of structural equation modeling (SEM) based on PLS. Research proves that role conflict and job stress have a negative and significant effect on job satisfaction, role conflict has a positive and significant effect on job stress and job stress is able to mediate the effect of role conflict on job satisfaction. Research proves that Gianyar Regency needs to increase employee job satisfaction, especially in increasing their PPNPN interest in carrying out their assigned work, needs to pay attention to the way employees do their work, and needs to pay attention to communication between employees and superiors. Keywords: job satisfaction, role conflict, job stress