Penelitian ini bertujuan untuk mengetahui berat molekul dan sebaran protein reseptor Growth Hormon (GHR). Sampel yang dipakai adalah otot rib eye sapi bali dan sapi Wagyu. Pemeriksaan berat molekul protein reseptor memakai metode SDS-PAGE, Western Blotting, dan pengecatan Imunohisto kimia. Hasil penelitian menunjukkan, bahwa berat protein reseptor Growth Hormon adalah 76 Kdalton pada kedua jenis sapi tersebut dan rerata nilai IHK-Score untuk otot sapi wagyu adalah 234,5 dan pada sapi Bali adalah 199,6. Dapat disimpulkan, bahwa berat molekul protein reseptor Growth Hormon otot rib eye pada kedua sapi Wagyu dan sapi bali adalah sama dengan sebaran yang tidak sama.
This study aims to prove that the Moringa leaf extract can improve renal histological structure of diabetic Wistar rats. Wistar rats were divided into six groups, so that each group has four replications. K1 as the positive control was not given the extract, K2 was administered the extract dose 100 mg/kg, K3 was administered the extract dose 200 mg/kg, K4 was administered the extract dose 300 mg/kg, K5 was administered the extract dose 400 mg/kg, K6 was administered the extract dose 500 mg/kg. The administrations of extract were held for five weeks. Rats then sacrificed and the kidneys were taken for the histopathology preparations with Hematoxylin-Eosin staining (HE). The observations indicate fatty degeneration and necrosis of the kidneys. Kruskal Wallis test results of all treatments showed p = 0.001 in both fatty degeneration and necrosis (p <0.05).
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung maggot Black Soldier Fly (Hermetia illucens) sebagai pakan tambahan pada broiler (Gallus domesticus) terhadap berat badan, berat dan struktur histologi hati. Broiler yang digunakan sebanyak 24 ekor dibagi ke dalam empat kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) masing-masing P0=0%, P1=1%, P2=2% dan P3=3% tepung maggot dalam pakan. Penelitian dilakukan selama 21 hari, broiler berumur 14- 35 hari. Parameter yang diteliti meliputi berat badan, berat dan struktur histologi hati yang diambil pada akhir penelitian. Analisis data untuk berat badan dan berat hati dilakukan dengan ANOVA dan dilanjutkan dengan uji Duncan. Untuk mengetahui hubungan antara keduanya dilakukan uii regresi. Analisis terhadap struktur histologi hati dilakukan dengan uji Kruskal Wallis. Hasil penelitian menunjukkan bahwa pemberian tepung maggot 1%, 2%, dan 3% menyebabkan berat badan dan berat hati broiler lebih rendah dibandingkan kontrol. Hasil uji regresi.menunjukkan terdapat hubungan yang kuat antara berat badan dengan berat hati. Pada pemeriksaan histologi hati menunjukkan terjadi degenerasi melemak pada hepatosit baik pada kelompok kontrol maupun perlakuan, serta tidak terjadi perbedaan nyata antara kelompok perlakuan. Dapat disimpulkan bahwa pemberian tepung maggot dalam pakan dapat menurunkan berat badan dan hati broiler. Ada hubungan kuat antara berat badan dengan berat hati. Tidak ada perbedaan gambaran histologi hati broiler antara perlakuan dengan kontrol.
This study aimed to determine changes in testicular histopathological of the rat strain wistar diabetes mellitus experienced. This study used 24 white rats (Rattus norvegicus) strain Wistar which were divided into six groups: administration of Moringa oleifera leaves P1 (extract dose 0 mg/kg bw) , P2 (100 mg/kg bw) , P3 (200 mg/kg bw), P4 (300 mg/kg bw) , P5 (400 mg/kg bw) and P6 ( 500 mg/kg bw) . In the end of administration, the diabetic Wistar rats were sacrificed and their testes were taken to make the histopathological preparations using haematoxylin and eosin staining. The observed variables are fat degeneration and necrosis. The results show that moringa leaves can improve histopathology testis structure of diabetes mellitus white rats. Extract of Moringa leaves (Moringa oleifera) dose of 400 mg/kg body weight has the best effect repair damage to the histopathological testis.
There have been studies of the histological structure of male and female goat skins. The histological structure and histomorphometry were taken from two region of skin, which is the thorax and abdomen. The Samples were collected from 2-3 years old Etawah goat, and than was stained using a HarrisHematoxilin Eosin method. The data were analysis based on the region and sex. The results showed that the histological structure of Etawah cross breed consists of three layers were epidermis, dermis, and hipodermis respectively. The epidermis composed by stratum corneum, granulosum, spinosum, and basal. We were found keratin layer on the surface of epidermis. The dermis consists of papillare and reticulare stratum. That was found many sebaceous glands, sudorifera gland, pili muscle, hair follicles, blood vessels, and collagen fibers. The hypodermis layers of Etawah cross breed females skin mostly consists of the fatty tissue, whereas in males we have found thick and horizontally arranged connective tissue. The skin layer on male Etawah cross breed, thorax region thicker than the abdomen. The region and sex may have an effect on the histology structure and histomorphometry of Etawah cross breed skin.
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