Histopathological of Diabetes Mellitus White Rat Testicle After Given Moringa Leaf Extract

jannah, Raodatul; Setiasih, Ni Luh Eka; Suastika, Putu

doi:10.24843/bulvet.2018.v10.i02.p11

Cited by 6 publications

(9 citation statements)

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“…The results of this study are in line with the findings of research conducted by Jannah (2018), that the oral administration of Moringa leaf extract at a dose of 100 mg/kg BW, 200 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW and 500 mg. / KgBW for five weeks of testicular histopathological examination of white male rats with Diabetes Mellitus showed an improvement in the histopathological picture of the testes after giving Moringa leaf extract (Jannah,2018). This is because Moringa leaf extract contains antioxidants that can help prevent damage caused by free radicals.…”

Section: Descriptive Analysis Of the Microscopic Image Of Mice Semini...supporting

confidence: 91%

The Effect of Moringa oleifera Extract on Histopathological of Mice Testes Exposed by Monosodium Glutamate

Handoyo,

Saraswati,

Mochtar

et al. 2023

dmj

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Background: Monosodium glutamate (MSG) consumption in excess can have an impact on a man's fertility and lead to infertility. MSG has the potential to produce free radicals, which can harm cells. Antioxidants can fight off free radicals. Exogenous antioxidants are required to maintain equilibrium since there will be an imbalance if the body produces more free radicals than endogenous antioxidants. Exogenous antioxidants flavonoids are present in the leaves of the Moringa plant (Moringa oleifera L). It is thought that flavonoids have 4-5 times more antioxidant potential than vitamins. Objectives: To evaluate how moringa leaf extract affects the histopathological profile of MSG-exposed mouse testes. Methods: Only the posttest was used as a control group in this study. 25 male mice were used as the sample, and they were split up into five groups. The treatment group 1 (P1) received 6 g/day of MSG exposure + 300 mg/kg BW/day of moringa leaf extract, the treatment group 2 (P2) received 6 g/day of MSG exposure + 600 mg/kg BW/day of moringa leaf extract, and the treatment group 3 (P3) received 6 g/day of MSG exposure + 1200 mg/kg BW/day of moringa leaf extract. The control group (K-) received only standard feed. The mice were put to death after 30 days. The testes were removed for histological examination using hematoxylin-eosin staining, and the Johnsen scoring criteria were applied to their evaluation. Results: The data were not significant according to the Shapiro-Wilk test in the P3 group (MSG 6 g/day + Moringa Extract 1200 mg/kg BW/day). The Kruskal-Wallis test revealed that there was no significant difference between the seminiferous tubules according to the treatment group (p = 0.117). Conclusion: Moringa oleifera leaf extract protects the histopathological picture of the testes of male mice compared to the group given monosodium glutamate.

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Section: Descriptive Analysis Of the Microscopic Image Of Mice Semini...supporting

confidence: 91%

The Effect of Moringa oleifera Extract on Histopathological of Mice Testes Exposed by Monosodium Glutamate

Handoyo,

Saraswati,

Mochtar

et al. 2023

dmj

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“…The decrease in inflammation in these cells is almost similar to a study conducted by Kusumawardani (2019), who found a decrease in the degree of pulmonary vasculitis in lupus mice model given MO leaf extract. Jannah et al (2018) also found that administering ethanolic extract of MO leaves had an anti-inflammatory effect on the testes of rats with diabetes mellitus at a dose of 400 mg/kg BW with histological improvements, namely, fat degeneration in the testes [ 17 ]. El Morsey et al (2019) also proved this anti-inflammatory effect.…”

Section: Discussionmentioning

confidence: 99%

Lacrimal Gland Histopathology and Secretory Function in Sjögren’s Syndrome Mice Model Treated with Moringa oleifera Lam. Leaf Extract

Susanto

Purwanto

Mudigdo

et al. 2021

AIAAMC

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Background: The pathogenesis of Sjögren’s syndrome involves the activation of NF-κB, producing proinflammatory cytokines such as tumor necrosis factor-α, interleukin (IL)-1α, IL-1β, IL-6, IL-17, and interferon-γ. Through oxidative stress, they will cause necrosis and apoptosis of lacrimal gland cells, resulting in impaired secretory function or reduced tear production. Moringa oleifera leaf extract is known to have strong anti-inflammatory and antioxidant activities. Objective: To determine the effect of Moringa oleifera leaf extract on lacrimal gland histopathology and secretory function in Sjögren’s syndrome mice model. Methods: The experimental study had a post-test only control group design with 32 eight-week-old male mice of the BALB/c strain divided into four groups, negative control (C−), which was not induced by SS, positive control (C+), treatment 1 (T1), and treatment 2 (T2) induced by Sjögren’s syndrome by immunizing with the 60-kD Ro antigen (SSA) as much as 100 µg. After 42 days, the T1 group was given dexamethasone 1.23 mg/kg BW/day orally for 14 days, whereas T2 was given dexamethasone 1.23 mg/kg BW/day and Moringa oleifera leaf ethanol extract 200 mg/kg BW/day orally for 14 days. At the end of the study, lacrimal gland histopathology and secretory function (tear production) were examined. Statistical analysis using F ANOVA/Kruskal–Wallis was followed by partial difference test with the Least Significant Difference post hoc test/Mann–Whitney. Significant if p < 0.05. Results: The comparison of lacrimal gland histopathology in T1 (p = 0.044) and T2 groups (p = 0.020) obtained significant results (p < 0.05) when compared to C+. However, the comparison of tear production in T1 (p = 0.127) and T2 groups (p = 0.206) was not significant (p > 0.05) when compared to the C+ group. Conclusion: The administration of Moringa oleifera leaf extract 200 mg/kg BW for 14 days could significantly improve lacrimal gland histopathology but was not effective in increasing tear production in Sjögren’s syndrome mice model.

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“…Previous studies have also shown that Moringa oleifera leaves extract can significantly reduce ROS production in sperm 10 . Other studies reported that it is beneficial in improving the histological appearance of the testicle of Wistar rats with diabetes mellitus by reducing fat degeneration and necrosis 11 . The administration of Moringa oleifera leaves ethanolic extract can also decrease the expression value of TNF-α in metabolic syndrome models 12 .…”

Section: Introductionmentioning

confidence: 97%

Effect of Moringa Leaves Ethanolic Extract (Moringa oleifera, Lam.) on Testicles Histopathological Structure in Metabolic Syndrome Rat Model

Aji,

Setyawan,

Hakim

et al. 2023

SMedJour

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Introduction: Metabolic syndrome was a collection of various disorders that can significantly increase the risk of experiencing insulin resistance, atherosclerotic cardiovascular disease, vascular complications, and diabetes mellitus. The detrimental effect of this condition has led to the development of various treatments options, such as Moringa leaves extract (Moringa oleifera), which was suspected to have ameliorative effects. Therefore, this study aimed to determine the effect of Moringa leaves extract on the histopathological appearance of the testes of Wistar rats induced with metabolic syndrome.Methods: This was an experimental design study, with a post-test-only control group, involving 30 Wistar rats (Rattus norvegicus). Metabolic syndrome was induced in the samples through the administration of high-fat and fructose feeds along with streptozotocin and nicotinamide. Furthermore, the moringa leaves extract was administered to the treatment groups in different doses of 150, 250, and 350 mg/kg BW. Testicular histopathological damage scores were then assessed on each group. The Kruskal-Wallis test which followed by Mann-Whitney test was used to determine the significant differences between the groups, while the correlation was analyzed using Spearman test.Results: There were significant differences in the histopathological scores between the treatment and control groups. Furthermore, the Wistar rats treated with Moringa extract showed better histopathological scores (2.1±0.64) compared to those without the treatment (2.7±0.54), p<0.05. The results also showed that higher doses of the extract significantly correlated with better outcome in testicular histopathological structure of metabolic syndrome rat model (p=0.000, r=-0.396).Conclusion: Moringa leaves ethanolic extract treatment improved testicular histopathological appearance in Wistar rats induced with metabolic syndrome.

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Histopathological of Diabetes Mellitus White Rat Testicle After Given Moringa Leaf Extract

Cited by 6 publications

References 1 publication

The Effect of Moringa oleifera Extract on Histopathological of Mice Testes Exposed by Monosodium Glutamate

The Effect of Moringa oleifera Extract on Histopathological of Mice Testes Exposed by Monosodium Glutamate

Lacrimal Gland Histopathology and Secretory Function in Sjögren’s Syndrome Mice Model Treated with Moringa oleifera Lam. Leaf Extract

Effect of Moringa Leaves Ethanolic Extract (Moringa oleifera, Lam.) on Testicles Histopathological Structure in Metabolic Syndrome Rat Model

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