Hospital is an industry engaged in health services which is responsible for health services. The purpose of this study was to analyze the effect of work-family conflict and work stress on the performance of female nurses in Wangaya hospital, Denpasar City. The data collection method in this study used a questionnaire. The questionnaire was addressed to 100 respondents for further analysis using classical assumption tests and multiple linear regression analysis. The results of the analysis showed that Work-Family Conflict had a negative and significant effect on the performance of female nurses at Wangaya Hospital, Denpasar City with work stress having a significant effect on nurse performance. women in Wangaya Hospital Denpasar City, and Work-Family Conflict (x1) and Work Stress (x2) have a significant effect on the performance (y) of Wangaya Hospital Denpasar City.