Gladiolus is an important estimated 8th in the world cut flower trade's cut flower grown throughout the world for its elegant attractive spikes of different hues and good keeping quality. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. However, multiplication rate of corms and cormels is slow and the conventional method of propagation is insufficient to meet the demand of planting material and eventually affect the final cost of corms. A number of improved conventional techniques including division of the corms, removal of leaf and flower spikes, use of standard corm size, and mechanical removal of sprouts can increase the multiplication rate of corms and cormels. These improved conventional methods of propagation are insufficient to meet the demand of planting materials. In vitro techniques are applicable for the propagation of corm producing species. These techniques are adopted at commercial level in order to fulfill supply gap of huge demand. A number of in vitro protocols have been developed for regeneration of Gladiolus plantlets using different media by using various explants sources of the plant. However, literature is rather scanty on in vitro cormel formation and acclimatization of in vitro propagules.