Increasing seeding or N rates provides negligible benefits in producing fine fescue sod.2. There are differences in sod strength and handling among fine fescue taxa.3. Strong creeping red fescue is a promising solution for low-input sod production.4. Higher N rates are required for Kentucky bluegrass compared to strong creeping red fescue.5. Tall fescue sod with no netting consistently provided poor sod strength and handling.

Research is required on sod to explore the differences in management and postharvest practices to help inform sod producers and reduce information inefficiencies that affect the supply and demand of cool-season turfgrass sod. Replicated field experiments were conducted in Indiana and Minnesota to quantify the effect of turfgrass species (strong creeping red fescue [Festuca rubra L. ssp. rubra Gaudin], Chewings fescue [F. rubra ssp. commutata Gaudin], tall fescue [F. arundinacea Schreb.], and Kentucky bluegrass [Poa pratensis L.]), seeding rate (1, 2, and 3 pure live seed (PLS) cm −2 ), and N fertilization (98, 196, and 294 kg N ha −1 yr −1 ) on growth rate and shelf-life storage. Turf height measurements occurred at multiple timings to calculate growth rates of treatments. Sod was harvested in the spring and autumn and stored on pallets for either 24 or 72 h and then reinstalled in its previous location and treatment effects were assessed. Seeding rate had no effect on growth rate or transplant success of sod. Differences in growth rates demonstrate potential savings for sod growers through less mowing requirement when producing fine fescue sod. Treatments did not influence internal heating of sod rolls, which were higher than, but similar to, diurnal fluctuations of air temperatures. There were minimal differences among turfgrass species and N rates, which indicates strong creeping red fescue, Chewings fescue, tall fescue, and Kentucky bluegrass sod stored for 24-72 h after a spring or autumn harvest in Indiana or Minnesota will not hinder transplant success.Abbreviations: DAI, days after installation; MAP, months after planting; PLS, pure live seedThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

The lack of turfgrass diversity in cool-season sod production is related to information inefficiencies, such as the lack of agronomic information available on improved, low-input species, such as fine fescues (Festuca spp.). Collaborative research between Purdue University and University of Minnesota investigated the influence of cool-season turfgrass species mixtures on the harvest (production and strength) and storage limitations of sod. Field experiments were established in 2018 in West Lafayette, IN, and in 2019 in St. Paul, MN. Treatments included 12 turfgrass species mixtures that consisted of the following five species: strong creeping red fescue (Festuca rubra L. ssp. rubra Gaudin), Chewings fescue (F. rubra ssp. commutata Gaudin), hard fescue (F. brevipila Tracey), tall fescue (F. arundinacea Schreb.), and Kentucky bluegrass (Poa pratensis L.). Data on turf cover, vertical growth rate, sod tensile strength, sod handling, and shelf-life storage and transplant success were collected at multiple harvest timings from 10 to 24 mo after planting. The majority of the sod mixtures, especially those containing ≥33% strong creeping red fescue, produced high quality sod similar to the standard comparison of 100% Kentucky bluegrass sod and transplant success was not severely affected when stored for 24-72 h immediately following a spring or autumn harvest. Sod mixtures containing tall fescue, including "rhizomatous tall fescue," consistently resulted in the lowest sod strength and handling. Overall, sod producers growing fine fescue sod may be able to not only reduce management inputs, but also yield good sod strength with low-input sod mixtures.

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Soybean breeding relies on the use of wild (Glycine soja Sieb. and Zucc.) and domesticated [Glycine max (L.) Merr.] germplasm for trait improvement. Soybeans are selfpollinating and accessions can be maintained as pure lines, however within-accession genetic variation has been observed in previous studies of some landraces and elite cultivars. The objective of this study was to characterize within-line variation in the accessions housed in the USDA Soybean Germplasm Collection. This collection includes over 20,000 accessions, each previously genotyped using the SoySNP50K Chip. Each SoySNP50K genotype was developed by pooling approximately three individuals per accession. Therefore, clusters of SNPs called as heterozygous within an accession can be inferred to represent putative regions of heterogeneity between the three individuals sampled. In this study, we found high-probability intervals of heterogeneity in 4% of the collection, representing 870 accessions. Heterogeneous loci were found on every chromosome and, collectively, covered 98.4% of the soybean genome and 99% of the gene models. Sanger sequencing confirmed regions of genomic heterogeneity among a subset of ten accessions. This dataset provides useful information and considerations for users of crop germplasm seed banks. Furthermore, the heterogeneous accessions and/or loci represent a unique genetic resource that is immediately available for forward and reverse genetics studies.