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There is a growing interest in the issue of inoculation of rhizobacteria into the agricultural soil because this group of bacteria can  increase  productivity and quality of  agriculturally important crop and contributes to the stability of agroecosystems. The aim of our work was to isolate and characterize of plant growth promoting traits (production of IAA, siderophores, phosphate solubilisation, antifungal activity) of rhizobacteria belonging to a group of plant growth promoting rhizobacteria (PGPR), and isolated from rhizosphere of maize (Zea mays L.) in luvisols.  Quantitative representation of rhizobacteria of maize was 7.4 . 106 CFU.g-1 dry soil. A total of eleven species of maize rhizosphere where isolated and confirmed as PGPR in vitro. The  all  isolates  showed positive indole-3-acetic acid (IAA) production ranging between 1.39 and 15.74 µg.ml-1. Seven  strains (63.6  %) has been shown with low and 1 strain with intermediate solubilisation index of phosphates and the  positive production of siderophores showed  7 isolates (63, 6 %). Except for the isolate KmiJP17B089, all others inhibited the growth of Sclerotinia sclerotiorum and Rhizoctonia solani by more than 50 %. In the case of Fusarium graminearum, on the other hand, we observed a very low inhibitory activity. The most active  in observed traits were  three isolates identified by the 16S rRNA gene sequencing and NCBI database using the BLAST  resource as Bacillus altitudinis strain KmiJP17B089, Bacillus aryabhattai strain KmiJP17B090  and  Bacillus megaterium strain KmiJP17B091. These results suggest the possibility to tested in vivo these Bacillus species as potential biological fertilizer  to increase maize production.

Species of the genus Beauveria are entomopathogenic fungi used in biological control to protect against insect pests. Identification of species is based on observation of morphological, phylogenetic and genetic differences. Currently, MALDI-TOF (Matrix Assisted Laser Desorption/Ionization-Time of Flight) mass spectrometry is often used in microbial diagnostic but identification of Beauveria species has not been tested before. The aim of this work was the possibilities of this method and to compare it with genetic analysis. Twenty strains of B. bassiana and B. pseudobassiana isolated from insect cadavers, mycoinsecticides and soil samples were used. Sequences of ITS (Internal Transcribed Spacer) region were used for genetic identification of strains. Sequences and results from protein analysis of isolates were compared with the reference strains of B. bassiana and B. pseudobassiana. Results of MALDI Biotyper softvare identification were correct for all strains. Identification was reliable with high log score (>2.1) for most of strains. Score value less than 1 was observed only in a single case -strain GHA isolated from mycoinsecticide Botanigard.The result of both analyses was also dendrogram (phylogenetic tree) which showed high degree of result consistence. MALDI-TOF identification of fungi from Beauveria genus is usable for fast and reliable identification of entomopathogenic fungi isolates acquired from environment and results can be utilized in conservation biocontrol strategies in agriculture and forest ecosystems. Keywords:Beauveria, genetic analysis, spectrum analysis of proteins