®A hamster model was used to determine the protector capacity of vax-SPIRAL against the lethal infection after challenge with 10,000 LD of four strains isolated of a leptospirosis outbreaks in Nicaragua. These strains were 50 selected from a primary group of 16 belonging to the serogroups strains Ballum, Icterohemorragiae, Pomona, Serjoe and Pyrogenes. Most of these, except Pyrogenes serogroup, reproduced the infection in animal model and the most virulent representatives of each serogroup were selected for the challenge. The results of this study ® suggest that vax-SPIRAL conferred high percents of heterologous protection which aimed to consider it as a prophylactic measure useful in situations of floods and natural disasters in Nicaragua.