Recently, a new phytoplasma was discovered in Hillsborough County in the state of Florida, USA. This phytoplasma belongs to the 16SrIV taxonomic group and is classified as subgroup D. It is the causal agent of lethal bronzing disease (LBD) of palm. Since the discovery of LBD in 2006, the disease has spread throughout much of the state. In 2014 and 2015, stands of cabbage palm and queen palms that had been present at the University of Florida's Fort Lauderdale Research and Education Center in Davie, FL began showing symptoms of LBD. After confirming the presence of the LBD phytoplasma in initially infected palms by nested PCR and RFLP analysis, all palms were systematically sampled over the period of 1 year to monitor and quantify disease spread. A total of 30 cabbage palms were tested monthly by qPCR, with five testing positive on the first sample date. By the end of the study period, 16 cabbage palms had died from the infection. A total of 16 queen palms were surveyed, with three palms initially testing positive. By the end of the study, four queen palms had tested positive and died from the infection. To the authors' knowledge, this study is the first to document and quantify spread of palm-infecting phytoplasmas. This data provides important insights into the ecology of palm-infecting phytoplasmas and highlights the impact that the movement of infective insects can pose to established stands of palms.

The 16SrIV-D phytoplasma was first identified in Florida in 2006. Since its discovery, it has spread throughout most of the state. It is most prevalent in the central part of Florida, from Hillsborough County on the west coast to St. Lucie County on the east coast. The 16SrIV-D phytoplasma is the causal agent of lethal bronzing disease (LBD), which is also known as Texas Phoenix palm decline (TPPD). It affects a variety of common and economically important ornamental palm species as well as the native and ecologically important species, Sabal palmetto. It has spread into the southern portions of Florida, where the palm species diversity is higher. The aims of this survey were to document the spread of disease in terms of geographic and host range one decade after its introduction into Florida, and to assess the risk that LBD poses to the nursery and landscaping industries. The survey included samples received from stakeholders throughout the state, covering 18 counties, as well as a systematic sampling of palms at the Fort Lauderdale Research and Education Center (FLREC), where the disease is spreading actively. The findings of this survey resulted in the detection of LBD in eight new counties, including Collier, Hernando, Jefferson, Martin, Miami-Dade, Monroe, Seminole, and St. Johns, and the expansion of LBD into four new host species, Cocos nucifera, Livistona chinensis, Butia capitata, and Carpentaria acuminata. These findings are crucial for stakeholders because they highlight new hosts of 16SrIV-D phytoplasma and the geographic expansion of the disease, indicating that vigilance is needed when surveying declining palms.

Lethal bronzing disease is caused by the 16SrIV-D phytoplasma and is fatal to many palm species. This disease has caused significant economic losses to nursery and landscaping industries in Florida. As is the phloem-limited nature of phytoplasmas, the lethal bronzing disease phytoplasma is transmitted only by phloem-feeding insect vectors. However, the vector remains unconfirmed and this impedes the development of Integrated Pest Management programs that target vector populations. The primary objective of this study was to identify potential vectors of the 16SrIV-D phytoplasma in Florida by surveying auchenorrhynchans using sticky traps at the area where the disease is actively spreading, and screening the specimens for the presence of the phytoplasma. Cixiidae, Cicadellidae, Derbidae, and Membracidae insects were collected consistently during the 1-yr survey. The total number of a cixiid planthopper, Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae), was significantly higher than other insects that were collected on the traps. Of 3,734 specimens tested by nested polymerase chain reaction, approximately 0.672% of the H. crudus specimens and 1 unidentified leafhopper (Cicadellidae sp.) tested positive for the16SrIV-D phytoplasma. The 16S sequences amplified from the positive specimens were confirmed by sequencing and phylogenetic analysis. Based on the population survey and the polymerase chain reaction assay results, we suggest that H. crudus is the potential vector of the 16SrIV-D phytoplasma. However, the vector competency of H. crudus needs to be further investigated due to the possibility of feeding residue being detected. The study has important implication for current lethal bronzing disease management by monitoring and controlling vector candidates in disease areas.

Lethal bronzing disease (LBD) is a fatal infection in a variety of ornamental palms in Florida caused by the 16SrIV-D phytoplasma. The disease was first found in 2006 in Florida and has since spread to 31 different counties, reaching as far north as Jacksonville and as far south as Key Largo. Stakeholders (nursery personnel, landscaping personnel, and extension agents) from across the state take and send samples for analysis. To provide better sampling recommendations and reduce the time associated with the sampling process, the distribution of the 16SrIV-D phytoplasma was examined by quantitative PCR analysis in various Phoenix sylvestris that displayed different stages of decline, including early symptoms, moderate symptoms, and late symptoms. A declining Sabal palmetto was also available for analysis and examined as well. The findings of this study revealed that regardless of the stage of decline, the highest amount of phytoplasma detected was nearest to the base of the trunk and gradually decreased further up the trunk. Also, in P. sylvestris it was found that with symptoms present, the entire trunk has a systemic infection, resulting in positive reactions for every sample taken. These data are useful to stakeholders because they provide useful sampling recommendations in that only a single sample is needed when sampling a palm suspected to be infected with phytoplasma. Also, based on these results, sampling lower on the trunk is advised.