Pisang talas (Musa paradisiaca var. sapientum L.) mempunyai nilai jual yang tinggi. Perbanyakanbibit pisang talas dilakukan menggunakan anakan hanya berjumlah 5-7 anakan per tanaman. Olehsebab itu, perbanyakan bibit dilakukan dengan kultur jaringan. Penelitian ini bertujuan untukmengetahui pengaruh modifikasi media dengan periode subkultur terhadap pertumbuhan pisangtalas melalui kultur jaringan. Penelitian ini didesain menggunakan Rancangan Acak Lengkap (RAL)faktorial dua faktor. Faktor pertama adalah modifikasi media yaitu: media Murashige Skoog (MS)(m1), MS + BAP 0,5 mg l-1 (m2), MS + TDZ 0,04 mg l-1 (m3), MS + TDZ 1,5 mg l-1 + BAP 1,0 mg l-1 (m4),½ MS + TDZ 1,5 mg l-1 + BAP 1,0 mg l-1 (m5), dan ½ MS + vitamin modifikasi (m6). Faktor keduaberupa periode subkultur, yaitu 6 minggu setelah tanam (mst) (s1) dan 10 mst (s2). Hasil penelitianmenunjukkan bahwa media MS dengan periode subkultur 10 mst dapat memberikan saat muncultunas tercepat yaitu 4,67 hari setelah subkultur (hss), media MS + BAP 0,5 mg l-1 dengan periodesubkultur 10 mst memberikan saat muncul akar tercepat yaitu 8,17 hss. Media MS + BAP 0,5 mgl-1 menghasilkan jumlah tunas sebanyak 2,75 buah dan media MS menghasilkan jumlah akarsebanyak 3,25 buah.Kata kunci: pisang talas, media, subkultur

Durian lahung is a type of endemic durian to Kalimantan which has a unique characteristic, namely its red skin. The existence of this plant is almost extinct so that conservation needs to be done and one way is to use tissue culture techniques. As first step, it is necessary to conduct experiments to obtain the right sterilization method in order to obtain aseptic planting material. This study aims to determine the effectiveness of sterilant formulation on type of explant for lahung durian culture. This study was designed using two factors factorial Completely Randomized Design (CRD) and was repeated four times. The first factor was formulation of sterilant which consists of five levels and the second factor was type of explant which consists of two levels, i.e leaf and node. The results showed that the combination of sterilant formulation tween 20, fungicide, bactericide, Bayclin 20%, alcohol 70%, HgCl2 0,05%, H2O2 17,6% with leaf explant produced the highest percentage of browning until 4 wap. There was no browning in all combinations of sterilant formulations with node explant. The sterilant formulation tween 20, fungicide, baktericide, alcohol 70% gave the longest time to appear contamination and browning, smaller percentage of contamination and greater percentage of live explants at 2 and 3 wap, while at 4 wap it was obtained from the sterilant formulation tween 20, fungicide, bactericide, Bayclin 20%, alcohol 70%, HgCl2 0,05%. Leaf explants had lower percentage of contamination and higher percentage of live explants than node explants.

This study aims to determine the effect of interaction between several sterilants and types of explants and determine the best interaction with stevia culture's success rate. This study was designed using a Factorial Complete Randomized Design with two factors and three replications. The first factor is sterilant formulas i.e: fungicide 3% + alcohol 70% + Bayclin 5%; fungicide 3% + bactericide 6% + 70% alcohol + Bayclin 5%; sublimate 0.1% + 70% alcohol + Bayclin 5%; and fungicide 3% + bactericide 6% + sublimate 0.1% + alcohol 70% + Bayclin 5%. The second factor is explant types, i.e., stevia nodes and leaves. The variables observed are the percentage of contamination (%), percentage of alive explants (%), and browning percentage.  The interaction between sterilants and explant types only had a significant effect on the percentage of alive explants. The best interactions of sterilant formulas and explant types on the percentage of alive explants are 0.1% sublimate + 70% alcohol + 5% Bayclin and leaf explants.

<p align="center"><strong>ABSTRACT</strong></p><p>Talas banana (<em>Musa paradisiaca</em> var. <em>sapientum</em> L.) has a high economic value. However, because of slowly breeding, a quick way of propagation: i.e. in vitro is required.Peatlands inSouth Kalimantan are quite large and has potential to be used for planting area of Talas banana. The acclimatization of seedlings planted on the peatsrequires more fertilizers, considering the low nutrient content of the peats. This study aims to determine the chicken manure and nitrogen-phosphor-potassium (NPK) fertilizer on the growth of Talasbanana acclimatized in peatlands. This study applied a randomized block design (RBD) factorial: (1) the amount of chicken manure (K), namely: k₁ = 5 t ha^-1; k₂ = 10 t ha^-1; k₃ = 15 t ha^-1; k₄ = 20 t ha^-1, and (2) the amount of NPK fertilizers (P), namely: p₁ = 200 kg ha^-1; p₂ = 250 kg ha^-1; p₃ = 300 kg ha^-1. Therefore, 12 treatments. DITAMBAHKAN TERDAPAT 12 PERLAKUAN. All treatments have 3 replicates. The results of the experiment showed that there was no interaction between doses of chicken manure with NPK. The amount of chicken manure 5 t ha^-1 resulted in the highest increment of plant height observed at 2^nd, 4^th, 6^th, 8^th, 10^th and 12^thweek after planting, increment of stem diameter, addition of leaf number, width and length of leaves.The results of experiment also revealed that the best increment of plant height observed at 8^th week after planting and stem diameter observed at 4^th week after planting was obtained by 250 kg ha^-1of NPK fertilizer application. KOMBINASI TIDAK BERPENGARUH</p>Key-word: propagation; in vitro; peatlands; chicken manure; Talas banana