Aims: Endophytic fungi are the remarkable category of host-associated fungal community that invades the intercellular regions of host tissues, benefiting their host while obtaining an advantage. Fungal endophytes have lately attracted prominence as a source of active secondary metabolites. This investigation aimed to identify fungal endophytes that reside inside the leaves and stems of Aquilaria malaccensis. Methodology and results: Healthy A. malaccensis stems and leaves samples were collected. Clean leaves and stems were cut to a size of 1 cm, followed by sterilization using 75% ethanol for 1 min, 3% sodium hypochlorite solution for 1 min, and finally, rinsing with sterile water 3 times for 1 min and drying with sterile paper. The sterile samples were put onto Potato Dextrose Agar (PDA) media containing chloramphenicol for 7-14 days until the mycelium grew for morphological identification under a light microscope. Five endophytic fungi were recovered from leaves, while nine endophytic fungi were obtained from stems. Using morphological approaches, nine of the endophytes had observed to produce conidia fungi, whereas the others did not. Neopestalotiopsis sp., Aspergillus sp., Arthrinium sp., Curvularia sp., Podospora sp., Mucor sp. and Verticillium sp. were identified as nine of the fourteen endophytes. Conclusion, significance and impact of study:The number of endophytic fungi discovered in different organs varies. Not all endophytic fungi that grow can create sexual phages. Six genera of endophytic fungi were identified.
Vitamin C is a nutrient that the body needs in order to increase its metabolism. This helps the body to create collagen and also act as an antioxidant. This study aims to analyze of vitamin C and the antioxidant activity of Capsicum frutescens L. and Capsicum annuum L. (curly and large chili variety), obtained from the Pinrang Regency, South Sulawesi Province. The study was carried out experimentally by UV-Vis spectrophotometry method. The measurement of vitamin C was carried out at a wavelength of 265.667 nm while for the antioxidant activity test the maximum wavelength of DPPH was 515.961 nm. Vitamin C levels obtained in Capsicum frutescens L. and Capsicum annuum L. (curly and large chili variety), were 0.926±0.75; 0.344±0.35, and 0.281±0.22±0.22% w/w. For antioxidant activity, the results IC50 of Capsicum frutescens L. and Capsicum annuum L. (curly and large chili variety), were 2.202; 2,260; and 2.751 ppm while the comparator vitamin C was 12.360 ppm. These results indicate that Capsicum frutescens L. has higher levels of vitamin C than Capsicum annuum L. (curly and large chili variety), while for the antioxidant activity test, the IC50 value of Capsicum frutescens L. and Capsicum annuum L. (curly and large chili variety) less IC50 value antioxidant activity than a comparison vitamin C, which means that they have strong antioxidant activity.
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