Objective: Viola odorata has a characteristic as antifungal, antibacterial, anticancer, antioxidant, antiasthmatic, anti-inflammatory, anti-HIV, and antipyretic agents. The aim of this study was detected about bioactive compounds in the methanolic extract of V. odorata. Methods:The methanolic extract was analyzed through gas chromatography-mass spectrometry (GC-MS) for the identification of different compounds. Results:The current study investigated about phytochemicals in flowers of V. odorata. GC-MS analysis of the methanol extract of flowers showed 84 compounds. The highest concentration was for components which include ethanol, 2-(9,12-octadecadienyl oxy) -, (Z,Z)-; pentadecanoic acid; 1-pentacosanol; 1-pentacosanol; 2-furan carboxaldehyde, 5-(hydroxymethyl)-; 1,2 benzenedicarboxylic acid, diisooctyl ester; and docosane, 11-butyl-and gamma-sitosterol. The peak area and retention time for each components, respectively, were (15.709, 25.51%), (14.015, 19.51%), (29.914, 4.69%), (27.292, 3.95%), (5.707, 4.05%), (20.357, 3.91%), (18.289, 2.48%), and (30.431, 2.37%). While the others components ranged the peak area from 2.03% to 0.05%. Conclusions:These results indicate that the flowers of V. odorata contain the numerous components which have medical importance and this study was one of the first studies to detect phytochemicals in V. odorata.

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Background:Within the past few decades, there has been an increase in the number of water-borne disease outbreaks and emergence of newly recognized waterborne parasites. Several factors which contribute to the spread of these diseases include: water, heavy rains and agricultural residues which transfer the parasites to water surface from the soil. The aim of this study was to detect the presence of parasites in the river and drinking water of Al-Wahdaa and Al-Rasheed Drinking Project and household water tanks from some regions of Baghdad.Method:Forty samples were collected from river and drinking water of Drinking Project. Fifty four samples of household water tanks were collected from some regions of Baghdad.Results:Cryptosporidium oocyst, which was founded in river water samples are more than those in drinking water. Furthermore, it was existed in Diyala Bridge &Taha Mosque from April – August and oocyst was diagnosed by using of Zheil-Neelson. Wet mount slide method was applied to detect cysts of free-living amoeba Acanthomoeba, Naegleria. The number of cysts in July and August were higher than other months for Drinking Project and the water tanks for all regions.Conclusion:These results emphasize the importance screening of the water to prevent possible of the spread of parasitic protozoan and that the cracks occurrence in drinking water pipes between the stations and houses led to contamination of water with the infective stage of parasites, especially in the areas that are near agricultural fields which polluted with the cows’ waste.

The intensity of Magnetic field by 200, 300 and 400 gaos were selective to study their impacts on bacteria Bacillus, Pseudomonas and yeasts Candida dubliniensis, Candida glabrata, Issatchenkia orientalis and Rhodotorula mucilaginosa growth and to reduce organic pollutants in wastewater by COD, TOC, TN and TP in concentrations of 180, 75, 52 and 84 ppm in pH 7.6 and treatment periods of 2, 4, 6 and 18 h in batch system. Results showed that magnetic field 300 gaos had higher ability to increase bacterial and yeasts growth by 400-600% in 18 h and reduced COD, TOC, TN and TP by 88, 85, 90 and 98.5% in same period treatment. While, the intensity of magnetic field 200 and 400 gaos have no effect on microorganisms growth and reducing organic pollutants. This study is first record for showing and explaining the positive effective of magnetic field on microorganisms growth.