Eruca sativa (jarjeer) is an annual herb (family Brassicaceae), which contains a wide range of chemicals and minerals with nutraceutical and organoleptic characteristics. Jarjeer was generally used as a food and traditionally mainly consumed due to its aphrodisiac properties. This crop known to contain various phytochemicals such as flavonoids, phenolic acids, terpens, carotenoids, tannins, glycosides, saponins, sterols, alkaloids, and other secondary metabolites. In leaves, kaempferol and its derivatives, glucosativin, are the main flavonoids and glucosinolate, respectively, while erucic acid and glucoerucin are the main fatty acid and glucosinolate, respectively. Medicinally, the plant has antibacterial, antidiabetic, antihypertensive, antiplatelet, and antioxidant activity and stimulates hair growth and other effects. Trails on topical pharmaceutical preparations involve the use of E. sativa which had been done. These preparations include creams and waxs which are intended to be used for potentiating hair growth and skin fungal and bacterial infection.
Objective: The aim of our study was to compare between flavonoids and phenolic acids contents of leaves and fruits of Melia azedarach since no phytochemical investigation had done previously in Iraq.
Methods:The leaves and fruits of Melia azedarach were extracted by soxhlet using 80% ethanol then the dried extract was suspended in water and fractionated using petroleum ether, chloroform, ethyl acetate, and n-butanol. The n-butanol fraction was hydrolyzed by acid and partitioned with ethyl acetate. The different fractions containing flavonoids and phenolic acids were analyzed by HPLC and HPTLC.
Results:The HPLC results revealed the presence catechin-7-O-glycoside in fruit only, while kaempferol-7-O-glycoside is found in the leaves only. Catechin and its glycosides are more abundant in the fruits than in the leaves. The HPTLC results revealed that kaempferol and quercetin are present in all fractions of leaves and fruits as aglycones and as glycosides. Free chlorogenic was found in both leaves and fruits.
Conclusion:No major differences were found between the flavonoids and phenolic acids contents of the leaves and fruits of Melia azedarach.
Gum Arabic is a natural gummy exudate gained from the trees of Acacia species (Acacia senegal and Acacia seyal), Family: Fabaceae. Gum Arabic considers as a dietary fiber with a high percentage of carbohydrates and low protein content. Sugars arabinose and ribose were originally discovered and isolated from gum Arabic and it is representing the original source of these sugars. A gum emanation from trees occurs under stress conditions such as heat, poor soil fertility, drought, and injury. Mainly gum is produced in belt region of Africa, mainly Sudan, Chad, and Nigeria. In the food industry, it is used in confectionery; in the pharmaceutical industry, it is used as emulsifier, film coating and others. Traditionally the gum used for chronic renal failure, digestive discomfort, and others. Although gum Arabic considered as an inert substance, recent information demonstrated multiple pharmacological and medical effects, such as weight reduction, antihypertensive, antihyperlipidemic, anticoagulant, antibacterial, antidiabetic, anti-inflammatory, nephroprotective and other effects.
Objective: The aim of our study was to investigate chemical constituents of leaves of Chenopodium murale since no phytochemical investigation had been done previously in Iraq.Methods: Leaves of C. murale were macerated in absolute methanol for 2 days and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate fraction was analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and coumarins contents. Coumarin derivative and phenolic acid were isolated from this fraction and identified by gas chromatography/mass spectrometry, infrared, ultraviolet, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of gallic acid and coumarin.Conclusion: The results of the current study showed the presence of scopoletin and gallic acid in the ethyl acetate fraction of C. murale.
Objective: the objective of this work was to investigate the phytochemical groups and to detect phenolic acids in L. camara leaves using highperformance thin-layer chromatography (HPTLC). Methods: Leaves of L. camara were macerated thrice a week in petroleum ether, then in ethanol. Powdered leaves and ethanolic extract were subjected to phytochemical investigation. Acidic hydrolysis were performed on the extract, and then fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The chloroform and ethyl acetate fractions were analyzed by HPTLC for their phenolic acid contents. Result: L. camara leaves devoid of alkaloids. Three different phenolic acids were detected in the leaf extract. Conclusion: Gallic acid, caffeic acid and p-coumaric acid were detected in L. camara leaves
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