Barn Owl (Tyto alba) pellets were collected from nine locations and two districts of Sindh, Pa�istan and 937 prey items were recovered from 619 pellets. Rats/mice (59.6%) were the most dominant food items consumed by the Barn Owl. Shrews (22.3%), bats (1.3%), birds (12.0%), insects (1.3%), frogs (2.2%) and plant materials (1.3%) were found in their diet as well. Study of the pelvic girdle bones of rats/ mice, used only for sexing, proved to be a useful device in population dynamics. In the pelvic bone, pelvic symphysis is found only in female rats/mice developed as a result of sex hormones that occur during gestation. Among the diet of rats/mice, males were found to be significantly dominant. Tooth wear patterns on the occlusal surfaces of molariform teeth of the rats/mice were found to provide an effective criterion for establishing age classes of rats/mice. In the present study, adult rats/mice were found to be dominant over sub-adults and old adults. ANOVA showed significant differences in the number of rats/mice and shrews (prey items) and the other prey items / plant materials in the diet of Barn Owls in the district Thatta and district Karachi. Chi-square test disclosed non-significant differences in age and sex categories.

Treatments offered in clinics are now ineffective due to antibiotic resistance in particular bacterial strains. Plant-based antibiotics are in high demand in developing and developed countries; they are common medications that are simple to use, pose no environmental risks, have no adverse side effects, and are competitively priced. This study aimed to screen plant-based medicine against Erythromycin-resistant bacteria such as E. coli, Enterobacter, Enterococcus, and E. Faecalis. The disk diffusion method and the agar well diffusion method were used to determine the zone of inhibition of Coriandrum Sativum L. (coriander), Zingiber officinale (ginger), and Spinacia oleracea (spinach). Minimum inhibitory concentration was evaluated via UV-visible Spectrophotometry at 600nm, while Polymerase Chain Reaction and Electrophoresis identified genomic activity for ErmB. Coriander was found to be the most effective against Erythromycin-resistant bacteria, and ErmB genes were found in almost all of the isolates. Keywords: Coriandrum Sativum, Zingiber officinale, Spinacia oleracea, Antimicrobial activity, and Erythromycin