Many plants are used in traditional medicine because they contain several molecules endowed with therapeutic activities. For this purpose, and in the framework of valorisation of the Algerian flora, our objective consists of the study of the chemical composition of two vegetable sources pushing for the spontaneous state in the region of Béchar (R. officinalis L. And J. phoenicea L.), and evaluation of the antibacterial activity by determination of the mini-mum inhibitory concentration (MIC) of the extracts on a set of pathogenic bacteria that are taken from the operating theater (EPH Tourabi Boudjamaa, Béchar, Algeria). The methanol/water (80:20, v/v) for 24h extracts were sub-jected to various phytochemical tests for the colorimetric assay, other frac-tions of methanol/water (80:20, v/v), acetone/water (80:20, v/v), and hot distilled water were used to evaluate the antibacterial activity (Disk diffusion and microdilution methods) against airborne pathogenic microorganisms. Phenolics compound contents in various extracts of R. officinalis and J. phoenecea are 2.12±0.14 and 2.64±0.2 mg GAE/g DW, respectively. The re-sults for flavonoids vary between 4.89±0.39 and 6.09±0,35 mg CE/g DW also respectively. For E. coli, the methanol/water extract of J. phoenecea has a stronger effect with a maximum inhibition of 19 mm in diameter, and 12 mm for for Gram positive (S. aureus). And with a low antibacterial effect of the extract of R. officinalis. The results we obtained in this study reveal the sensi-tivity of the various airborne microorganisms collected from operating thea-ters confirming the effectiveness of the methanol / water extracts of the various plants used especially of J. phoenecea.
Background: Worldwide different plants were studied for their role in the treatment and correction of different types of anaemia's. The Medicinal and Aromatic Plants Research Institute (MAPRI) of the National Centre for Research (NCR) plays major role in medicinal and aromatic plants investigation, determination of therapeutic properties and documented in Sudan.Objectives: This study used to determine the effect of Cajanus Cajan seeds extract on the some haematological parameters among hemorrhagic anaemia rats, Khartoum -Sudan.Methods: Experimental randomised controlled trial study conducted among 24 healthy Wistar albino rats with both sex, ages (6weeks) and weighing between (75-130g), they were feed with standard laboratory chow. they divided into four groups each one consists of six rats.Result: Dose group(800 mg/kg/day) represent highly significant increases in haemoglobin in W0&W1 P value < 0.001,and W0&W3 p value is 0.0002.while the dose of 400mg/kg/day significant increase in W0&W1 p value is< 0.003 and W0&W3 p<0.00004, in the dose of 200mg/kg/day represent also significant increase at W0&W1 p<0.005 and in W0&W3p<0.007 respectively, when compared to control values(Group1).
Conclusion:This study revealed that there are increased in some haematological parameters and lend support to the use of ethanol seeds extract of Cajanus Cajan in treatment of hemorrhagic anaemia.
The current study focuses on the phytochemical characterization and biological activity of phenolic compounds derived from leaves of Moringa oleifera Lam. (Moringaceae) in the Tabelbala area (Bechar, Algeria). For a concentration of 1 mg/ml, the antioxidant tests revealed that the different extracts have a good reducing activity, with the tannin extract having the highest percentage of free radical inhibition (DPPH: 94%) compared to the n-butanolic extract (92%) and the ethyl acetate extract (88%). Furthermore, the tannin extract and the n-butanolic fraction of the flavonoids of the examined leaves showed significant antioxidant activity compared to ascorbic acid used as a reference (IC50 = 0.05 ± 0.14 mg/ml), with IC50 values of 0.07 ± 0.79 mg/ml for tannins and 0.0823 ± 0.25 mg/ml for the n-butanolic extract. In addition, the ferric reduction antioxidant power (FRAP) test reveals that all the tested extracts have a significant reduction power. The different selective extracts showed strong antimicrobial activity against nine pathogenic microbial strains: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 13883), Citrobacter freundii (ATCC 29212), Acinetobacter baumannii (ATCC 19606), Bacillus cereus (ATCC 11778), Staphylococcus aureus (ATCC 25923), Enterococus faecalis (ATCC 13045), and Candida albicans, where the ethyl acetate fraction has a higher antibacterial activity than n-butanolic and tannin extract, with the zone of inhibition diameters ranging from 11 to 23 mm and have minimum inhibitory concentrations (MIC) between 0.39 and 3.125 mg/ml. These findings give scientific support for the plant’s traditional use, highlighting the value of traditional medicines in the treatment of many diseases.
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