We previously reported that phosphatidylinositol 4,5-bisphosphate (PIP 2 ) dramatically increases the gelating activity of smooth muscle ␣-actinin (Fukami, K., Furuhashi, K., Inagaki, M., Endo, T., Hatano, S., and Tak 168 -184) or the bacterially expressed protein (amino acids 137-259) were confirmed by enzymelinked immunosorvent assay. We also found this region homologous to the sequence of the PIP 2 -binding site in spectrin and the pleckstrin homology domains of PLC-␦1 and Grb7. Synthetic peptides from the homologous regions in spectrin and PLC-␦1 inhibited PLC activities. These results indicate that residues 168 -184 comprise a binding site for PIP 2 in ␣-actinin and that similar sequences found in spectrin and PLC-␦1 may be involved in the interaction with PIP 2

No abstract

To estimate the catch of Honmoroko Gnathopogon caerulescens by recreational anglers around Ibanaiko lagoon, which is adjacent to Laka Biwa, for eŠectiveˆsheries management, we randomly interviewed anglers from

No abstract

No abstract