Objective: Azelaic acid as an antibacterial to Propionibacterium acnes bacteria in recent years began to develop as an anti-acne using lipid bilayer vesicles to increase the penetration of azelaic acid to reach sebaceous tissue which is located in the dermis of skin where the growth of bacteria P. acnes. The development of azelaic acid in lipid bilayer vesicles is also due to the absorption of azelaic acid from cream preparations by only 4%. Azelaic acid gel based ethosomes also known give a larger zone of inhibition than azelaic acid creams and gels in the market.Methods: Preparation stability test of azelaic acid cream based ethosome was chemical and physical stability. The observed physics stability was organoleptic changes, homogeneity, pH, viscosity, cycling test, and mechanical test (centrifugal test). While chemical stability was performed to know the concentration of azelaic acid in cream to be periodically. Stability test was conducted for 12 weeks and observation every 2 weeks.Result: Physics stability test of that preparations was odorless, white, no change of phase, homogeneous, viscosity, and flow properties constant. While chemical stability test with the determination for 12 weeks to decreased content. However, these content is still included in the range of accuracy (90-110%). Conclusion:Azelaic acid of ethosome cream was physically and chemically stable.

Objective: Development of transdermal drug delivery systems has several advantages, especially drugs to have a poor penetration of stratum corneum in the skin. Azelaic acid has been proven bactericidal and bacteriostatic to acnes bacteria (Propionibacterium acnes). Azelaic acid products in market as cream and gel can only penetration in stratum corneum about 4% of the dosage used. Thus, it is necessary to increase the penetration of azelaic acid to formulate into a carrier system such as ethosome.Methods: Manufacture of suspension ethosom azelaic acid using thin layer hydration method or classical method. Suspension ethosom of azelaic acid to obtained subsequent freeze dried before formulated in cream preparation. After that, the penetration test for ethosom cream and non ethosom cream of azelaic acid with Franz Diffusion Cell.Results: Optimization formulation ethosom of azelaic acid with variations concentration of ethanol 30%, 35% and 40%. Ethosome with 35% ethanol had entrapment efficiency higher than 30% and 40% ethanol as 94.48±0.14% and had smaller particle size 179.3±2.23 nm. Penetration test for ethosome cream and non-ethosome cream of azelaic acid showed that cumulative amount was 1334.074±27.086 μg/cm2 h and 491.032±3.935 μg/cm2 h.Conclusion: Ethosome cream of azelaic acid has better penetration capabilities than non-ethosom cream of azelaic acid.