African Swine Fever (ASF) is a disease that infects pigs. Common symptoms caused by ASF attacks on pigs are bleeding in the feces and ears as well as sudden weakness of livestock, unable to stand, and death of livestock. ASF virus is not zoonotic so it does not affect human health. This virus has entered Indonesian territory, including the Nias Island, where most of the people cultivate local pigs. The ASF outbreak on local pigs in Nias resulted in the death of many pigs. This paper discusses the spread and death of local pigs due to ASF attacks in Nias. Data compiled from the field and the Animal Husbandry Office in 2020 in the Nias islands, it is known that the pigs that died due to the ASF outbreak reached 120,592 a pigs. Meanwhile, the fact found are that the handling of infected pigs cannot be done optimally, because until now, effective treatment and vaccination for the treatment and prevention of ASF infection has not been found. Therefore, in order to prevent the spread of the plague from becoming more widespread, the current methods that can be applied are the application of biosecurity in the cattle sheds, isolation of infected livestock and the role of the government in regulating the entry and exit route for pigs in the Nias region.

The present study aimed to determine the effect of cherry leaf extract (Muntingia calabura L) on hatchability of Balitnak Superior Free Range Chickens (KUB) through artificial hatching. The doses of cherry leaf extract used in this study were 0%, 10%, 15% and 20%. The hatching eggs used came from KUB hens that were intensively reared in the poultry house at UPBS BPTP, North Sumatra. The procedures applied in this study were collection of hatching eggs, fumigation of machines and equipment, setting of machines and hatching eggs, egg washing with cherry leaf extract and hatching of eggs for 21 days. During the hatching process, observations were made on predetermined variables. The observed variables were egg shape index, egg weight loss, shell temperature and hatchability. The method used was completely randomized design (CRD) with 4 treatments and 100 replications. The results showed that the hatching egg shape index in this study was 75,77 – 77,05%. The lowest egg weight loss was at P0 (0%), while the largest weight loss was at P2 (15%). The highest hatchability was observed in P1 (10%) at 84%. The most effective P1 treatment resulted in the highest egg hatchability in the present study.

This study was aimed to determine the germination process of seeds with different scarification treatments. This research was conducted at the Laboratory of Animal Nutrition, Faculty of Animal Science, Universitas Gadjah Mada, July 2019. The treatment in this research were different scarification methods of the seeds by sanding (P1), wounding (P2), soaking in H2SO4 (P3), soaking in warm water (P4), and baking at 55º C for 10 minutes (P5). Each treatment using 30 long bean seed that has been repeated for 3 times. Based on the five treatments, the seeds were planted in soil media and their growth observed for 14 days. The results showed that the seed growth for 14 days from the five treatments give an indication that the germination process and the first leaves grow out from long bean seeds was happen in treatment by oven 55º C (P5). Based on these results, it can be concluded that different scarification methods affected on the seed germination process. The fastest seed germination process to produce optimum growth in long bean plants was the scarification treatment by heating the seeds using an oven 55º C for 10 minutes (P5) before the seeds were planted.

This study was conducted to determine the effect of herbals on the production performance and gastrointestinal health of layer hens. There were 50 layer hens used and assigned randomly into two different types of diets namely, basal diet (T0) and basal diet+3ml herbals/1000g ration (T1) with each treatment consisting of 25 birds. Meanwhile, the herbals supplemented into the poultry feed consisted of tumeric, sambiloto leaves, soursop leaves, ginger, and lemongrass. Consequently, the T-test results showed that supplementation of herbals on treatment 1 (T1) statistically had no significant difference with treatment control (T0). The study concluded that the herbal mix could be used as a feed supplementation in a layer basal diet.

The purpose of this research is to compare the protein profile of herbals egg and non herbal egg using High Performance Liquid Chromatography (HPLC). This research used HPLC Shimadzu 6.1 with column C18, flow rate mobile phase 1 ml/min, wavelenght detector UV Vis 220 nm and temperature 50o C. Mobile fase that used in this research was 10 and 60 % acetonitril (CH3CN) in water containing 0,05% triflouroacetic acid. Supplementation of herb containing bioactive antioxidant compounds affect the formation of egg yolk protein containing immunoglobulin. In this study, herbal eggs and non-herbal eggs were seen from their protein profile using the high performance liquid chromatography (HPLC) method and then analyzed using descriptive qualitative analysis. The result show that herbal egg yolk sample has a dominant protein with a molecular weight of 50.41 kDa. Herbal egg yolk protein appears at a retention time (RT) of 56.87 minutes, an area of 3303488 units of area, and the peak height of the graph / peak at 50,974 µAU. Meanwhile, non-herbal egg yolk sample has a dominant protein with a molecular weight of 49.94 kDa. This protein appears at a retention time of 1.307 minutes, an area of 149445550 units of area, and the peak height of the graph / peak at 402.6026 µAU. The results showed that the the peak of HPLC indicated an antioxidants were bound to the bioactive protein fractions of egg yolk. It could be concluded that bioactive herbal bound to egg yolk igY, but the bioactive compounds have not been identified yet.