Elaeocarpus grandiflorus L. is an identity plant of Salatiga City. Wherein, the plant is difficult to naturally propagation. This study would obtain propagation technique of E. grandiflorus by tissue culture. It was experimentally conducted, consists of two sub-experiments. The first aimed to find the optimal sterilization agent was conducted with a completely randomized design, namely sterilizing compound. There were two compounds studied, i.e., NaOCl and Ca(OCl)2. The second sub-experiment aimed to determine the optimal medium type and antioxidant compounds for inducing in vitro shoots. It was conducted with a completely randomized design with two factors, namely the medium type [i.e. Murashige & Skoog (MS) and Wood Plant Medium (WPM)], and antioxidant agents (i.e. activated charcoal and polyvinyl pyrrolidone/PVP). Data were analyzed by descriptive percentage, analysis of variance, and least significant difference test. The results showed that Ca(OCl)2 and NaOCl were effective to obtain the sterile and alive explants. The MS media added by PVP resulted in the most explants forming shoots, and WPM supplemented with PVP or active charcoal caused most explants regenerating callus. The results could be used as a basis for the development of in vitro shoot multiplication to reach an efficient propagation of E. grandiflorus.

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