Healthcare professionals deal with the greatest prevalence of burnout syndrome (BS). The object of this paper is to study the effect of psychological climate and working conditions on risks of burnout in a team of healthcare professionals. Working conditions of 136 healthcare workers from the Saratov region who underwent little training were examined according to Regulation 2.2006–05. The level of burnout was estimated in accordance with the method of V. V. Boyko, and the psychological climate in a team was analyzed using A. F. Fiedler’s questionnaire. The results were processed with the help of Microsoft Еxcel 10 for Windows. Based on the research results, the BS signs were recorded in 52.3% of healthcare workers. Physical and emotional symptoms were predominant BS signs. Factors predisposing to BS in healthcare workers have been determined. It is established that there is a dependence between a harmful factor of working conditions and probable BS. The dependence acquires significance at 3.2 level of working conditions. It is determined that the risk of BS was 74.3% in the most unfavorable psychological climate, and didn’t reach 21% if the climate was favorable.