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Eggshell is a rich source of CaCO3 with a high purity content of more than 96.35% w/w and a potential raw material for calcium hydroxyapatite preparation. Mesoporous and nano‐particulate calcium hydroxyapatite was prepared from duck eggshell from the chemical reaction with phosphoric acid followed by the calcinations at 800, 900, and 1000°C for 2 hours. The optimum condition to obtain the high purity calcium hydroxyapatite was by sintering calcium phosphate Ca3(PO4)2 at 1000°C for 2 hours. The average particle size, pore diameter, specific surface, and true density of the sample sintered at 1000°C for 2 hours were 101.93 ± 12.15 nm, 98.96 Å, 2.12 m2/g, and 3.02 g/cm3, respectively, appearing as a soft fine powder with a white color. The raw duck eggshell is a potential candidate as a bio‐ceramic material to prepare calcium hydroxyapatite suitable for use in various bio‐applications such as bone tissue engineering, drug and gene delivery, remineralizing agent in toothpaste, and bone void fillers for orthopedic and restoration.

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Interest in the development of bio-based waste has increased exponentially in biomedical, biodental and bioengineering applications because such waste is nontoxic, biocompatible, easily formed, and has good physicochemical-mechanical-thermal properties, elasticity, high purity, no side effects and biodegradation. Eggshell membrane is a biopolymer network of protein fibers retaining albumin, collagen and amino acid. Eggshell membrane is a good food source for bacterial Acetobacter xylinum growth to make a strong biocellulose nanofiber composite for use in facial masks and tissue engineering. The current study added different amounts of eggshell membrane powder of 0% (Bio-0), 0.01% (Bio-01), 0.05% (Bio-05), 0.075% (Bio-075) and 0.10%wt (Bio-1) into biocellulose made from Acetobacter xylinum in coconut water medium via a fermentation process. The obtained facial mask encoded Bio-1 had good physicochemical and mechanical properties. The best sample has tensile strength, elongation at break and maximum force, and good adsorption  encoded Bio-1 equal to 3.229 ± 0.297 MPa, 43.350 ± 8.400% 13.466±1.238 N, respectively.