Interest in the development of bio-based waste has increased exponentially in biomedical, biodental and bioengineering applications because such waste is nontoxic, biocompatible, easily formed, and has good physicochemical-mechanical-thermal properties, elasticity, high purity, no side effects and biodegradation. Eggshell membrane is a biopolymer network of protein fibers retaining albumin, collagen and amino acid. Eggshell membrane is a good food source for bacterial Acetobacter xylinum growth to make a strong biocellulose nanofiber composite for use in facial masks and tissue engineering. The current study added different amounts of eggshell membrane powder of 0% (Bio-0), 0.01% (Bio-01), 0.05% (Bio-05), 0.075% (Bio-075) and 0.10%wt (Bio-1) into biocellulose made from Acetobacter xylinum in coconut water medium via a fermentation process. The obtained facial mask encoded Bio-1 had good physicochemical and mechanical properties. The best sample has tensile strength, elongation at break and maximum force, and good adsorption  encoded Bio-1 equal to 3.229 ± 0.297 MPa, 43.350 ± 8.400% 13.466±1.238 N, respectively.