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Background The quality of life in Class III malocclusion patients are worse than those without the disorder and previous studies have shown that surgical orthodontic treatment has a different effect on these patients compared with mild or moderate malocclusion. This study aimed to investigate the changes in quality of life in patients with Class III malocclusion during surgical orthodontic treatment in Chinese young adults. Methods The 14-item Short Form Oral Health Impact Profile (OHIP-14), and the 22-item Orthognathic Quality of Life Questionnaire (OQLQ) were used to assess the effect of surgical orthodontic treatment on the quality of life in 21 patients with Class III malocclusion at pre-treatment ( T 0 ), pre-surgical orthodontic treatment (6 to 8 months, T 1 ) and post-surgical orthodontic treatment (6 to 8 months after surgery, T 2 ), and 24 healthy individuals were included as controls. The comparisons in numerical variables between patients and controls were performed using Mann-Whitney U test. The scores of the two questionnaires between T 0 , T 1 , T 2 and controls ( T c) were compared using generalized estimating equation. Results According to OHIP-14 questionnaire, the mean scores in T 0 and T 1 were higher than those in T 2 and T c ( P < 0.001), and a significant decrease was observed after post-surgical orthodontic treatment ( P < 0.001), which achieved a level similar to the control group ( P > 0.05). As to OQLQ questionnaire, the mean scores of all domains showed a significant increase between T 0 and T 1 except for awareness of dentofacial aesthetics ( P > 0.05) and social aspects of dentofacial deformity ( P > 0.05), followed by a significant decrease between T 1 and T 2 . Conclusion Surgical orthodontic treatment may improve quality of life in patients with Class III malocclusion, but pre-surgical orthodontic treatment may have an adverse effect on quality of life.

Background: Blood contains exosomes that are related to tumor cells. Those exosomes can regulate communication between cells and have a great influence on a variety of tumor-associated proceedings through their target cells. Therefore, serum exosomes (SE) were supposed to play a crucial role in cancer development.Methods: This study presented a quantitative proteomics analysis to identify the protein content in SEs including 30 subjects from oral squamous cell carcinoma (OSCC) patients with lymph node metastasis (LNM), OSCC patients with no LNM (NLNM), and healthy controls (HC). Differentially expressed proteins (DEP) were analyzed by bioinformatics, and then a total of 30 subjects were used for Western blot and 60 subjects for IHC, ELISA, and RT-PCR verifications. The correlations were assessed between DEP expression and clinicopathologic factors.Results: A total of 415 proteins were identified. Comparing with HC and OSCC-NLNM, we found 37 proteins and 28 proteins in the SEs of OSCC-LNM, respectively. There were significant correlations among the expression of PF4V1 with tumor differentiation level, PF4V1 and F13A1 with the number of positive nodes, and ApoA1 with smoking and drinking. ROC curve analysis indicated that the combinations of the different biomarkers or specimen were obviously superior to single biomarker or specimen for diagnosing OSCC-LNM.Conclusions: We conclude that PF4V1, CXCL7, F13A1, and ApoA1 from SEs may be related to the metastasis of OSCC, which would be helpful in the diagnosis of OSCC-LNM.Impact: Biomarkers from SEs could help with the diagnosis of metastasis in OSCC.