This study aimed to produce scientific literacy instruments containing ethnoscience for grade IV elementary school on science subject theme number 9 "Kayanya Negeriku" which is valid, reliable, feasible, and practical. This research method used R&D research with ADDIE research design (analysis, design, development, implementation, and evaluation). The steps developed include design, expert validation, small-scale trials, and large-scale trials. The instrument developed is a reasoned multiple choice instrument. The data collection technique in this development research was a test (multiple choice) and a practicality questionnaire. The results of the validation of scientific literacy instruments containing ethnoscience are valid, the reliability value during small-scale trials was 0.671 and when large-scale trials were 0.711 these values indicate the instrument is reliable. The differentiating power of small-scale questions obtained 20 multiple-choice questions for grade IV elementary school, there were 10 items received, 2 items were accepted but needed to be corrected, and 8 items were corrected. The analysis of large-scale discrepancy from 20 questions contained 11 items for the criteria for the items to be accepted, 3 items were accepted but needed to be corrected, and 6 items were corrected. Testing results of the difficulty level gained the results of quantitative analysis on a small scale for the level of difficulty of the questions stated that, as many as 2 questions with easy criteria, 13 questions with medium criteria and 5 questions with difficult criteria. While the results of quantitative analysis on a large scale for the level of difficulty of the questions stated that, as many as 3 items with difficult criteria, 17 questions with moderate criteria and for easy criteria were not found in large-scale trials. The practicality of the instrument can be decided based on the percentage of practicality. The percentage obtained from the teacher's assessment is in very practical criteria. Keywords: Development, Instrument, Science Literacy, Ethnoscience.