The goal of this research was to determine the content of secondary metabolites and the most active fraction of Simbagh Utak (Hydnophytum sp.) Tuber extracts. The content of secondary metabolites is determined by using phytochemical tests, and toxicity tests are carried out by the Brine Shrimp Lethality Test (BSLT) method. Hydnophytum bulbs from Jukung Village, Lubuklinggau City, South Sumatra were macerated using 96% ethanol and then evaporated until thick extracts were obtained. This thick extract was then tested for secondary metabolite content, fractionated using n-hexane, and ethyl acetate. The results of the fractionation were then carried out a toxicity test using the BSLT method. Secondary metabolite test results showed ethanol extract containing flavonoids, alkaloids, tannins, saponins, terpenoids, and phenolics. The result of fractionation was obtained by the tuber extract of Hydnophytum n-hexane fraction, ethyl acetate fraction, and ethanol fraction. Toxicity test results obtained LC50 values of n-hexane fraction of 52.3 ppm, LC50 of ethyl acetate fraction of 45.9 ppm, and LC50 of ethanol fraction of 99 ppm. All Hydnophytum tuber extract fractions were categorized as toxic and have potential as anti-bacterial or anti-oxidant. The fraction that has the lowest LC50 price is the most toxic fraction. The most toxic fraction is the most active fraction. The ethyl acetate fraction was the most active fraction because it has the lowest LC50.

The National Economic Recovery (NER) Program is one of the responses initiated by the government in Indonesia's economic recovery due to the impact of COVID-19, the target is to reduce the activities of affected communities, including cooperatives. One of the priority aspects for the program to run well and smoothly is the role of institutions in knowledge management and process sharing. This paper examines the role of knowledge management and sharing in cooperatives with qualitative limitations at the knowledge process level, knowledge design level, strategic interaction level, social participation level, academic and scientific ecosystem level, and network and partnership level. A qualitative description becomes a research method with secondary data in the form of a comparison of cooperatives in 2019–2021 as a representation before and during the COVID-19 pandemic. COVID-19 secondary data for 20 months from April 2020 to September 2022 in Indonesia dynamically also support sharpening the analysis. The source of cooperative data is from the publications of the Ministry of Cooperatives and SMEs, while the source of COVID-19 data comes from the publication of the COVID-19 Task Force. The analysis is carried out by building qualitative aspects into quantitative ones that can be formulated in the form of cooperative applications. The result is that the application of the knowledge process level, knowledge design level, strategic interaction level, social participation level, academic and scientific ecosystem level, and network and partnership level can improve decision-making, capture, share, and measure institutional knowledge for the success of the NER Program.

Salak seeds have been developed as a beverage, but there was still a little amount of research that focused on salak seeds. This research was conducted to find out the chemical compounds and the antioxidant activity of ethanolic extract and fraction of salak fruits seeds ( Salacca zalacca (Gaertn.) Voss. ) which have been grown extensively in Sleman Yogyakarta. Extraction was conducted using maceration, followed by fractionation using vacuum liquid chromatography. The identification of the chemical compounds contained in the ethanolic extract and fraction was performed by thin layer chromatography method, while the antioxidant activity was performed by DPPH method. Comparison of antioxidant activity was seen using IC 50 values. The results showed that ethanol extract and fraction contained phenol, flavonoid, and tannin. The largest antioxidant activity was found in F7 with an IC 50 value of 110.16 μg / ml. Keywords: Antioxidant, salak fruit seeds, DPPH ABSTRAKBiji buah salak telah banyak dikembangkan sebagai minuman kopi biji salak, namun penelitian mengenai kandungan serta manfaatnya belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan dan aktivitas antioksidan ekstrak etanol serta fraksi biji buah salak pondoh ( Salacca zalacca (Gaertn.) Voss. ) yang tumbuh di Sleman Yogyakarta. Ekstraksi dilakukan dengan metode maserasi yang dilanjutkan dengan fraksinasi menggunakan metode kromatografi cair vakum. Identifikasi senyawa yang terkandung dalam ekstrak dan fraksi kemudian dilakukan melalui metode kromatografi lapis tipis, sedangkan aktivitas antioksidan dilakukan dengan metode DPPH. Perbandingan aktivitas antioksidan dilihat menggunakan nilai IC 50 . Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi mengandung fenol, flavonoid, dan tannin. Aktivitas antioksidan terbesar terdapat pada F7 dengan nilai IC 50 sebesar 110,16 µg/ml.

Pendahuluan: Tingginya masalah resistensi antibiotik di indonesia disebabkan karena penggunaan antibiotik yang tidak rasional sehingga diperlukan adanya antibakteri dari bahan alam yang dapat menghambat pertumbuhan bakteri dengan minimal efek samping. Salah satu bahan alam yang dapat digunakan sebagai antibakteri yaitu biji mangga arumanis (Mangifera indica L.Var. Arumanis).Sifat antibakteri pada biji mangga arumanis (Mangifera indica L.Var. Arumanis) disebabkan karena adanya senyawa fenolik berupa flavonoid, alkaloid, tanin, terpenoid, saponin, dan juga senyawa gallotanin.Tujuan:Untuk mengetahui aktifitas antibakteri ekstrak biji mangga arumanis dan bakteri apa saja yang dapat dihambat dengan mengetahui nilai MIC. Metode: Metode yang digunakan dalam penelitian ini yaitu metode PICO. Hasil: Dari literatur review biji mangga arumanis (Mangifera indica L.Var. Arumanis) terbukti dapat menghambat bakteri gram positif dan negatif dengan penghambatan lebih besar pada bakteri positif. Dengan nilai MIC sebesar 0.03mg/mL yang terbukti dapat menghambat bakteri Methicillin Resistan Staphylococcu aureus.Kesimpulan:Ekstrak biji mangga arumanis dapat menghambat bakteri gram positif dan negatif dengan nilai MIC dimulai dari 0.03mg/mL.

Pemenuhan gizi bayi usia 0-6 bulan merupakan suatu hal yang sangat pentingdan hanya dapat diperoleh melalui pemberian ASI Eksklusif, sehingga ibu menyusui membutuhkan tambahan nutrisi sebanyak 400-500 Kkal setiap harinya. Ibu membutuhkan tambahan kandungan nutrisi yang mampu meningkatkan produksi ASI seperti laktogogum dan flavonoid pada daun kelor dan biji fenugreek, akan tetapi Sebagian besar masyarakat belum mengetahui hal tersebut. Padahal Potensi sumber daya alam sangat melimpah dengan manfaat yang luar biasa seperti manfaat daun kelor dan biji fenugreek. Penelitian ini bertujuan untuk mengetahui perbedaan kecukupan ASI antara ibu yang mengkonsumsi ekstrak daun kelor (moringa oleifera) dengan ekstrak biji fenugreek (trigonella foenum-graecum) di wilayah kerja puskesmas linggang bigung. Desain penelitian ini adalah quasi  Experiment dengan rancangan post test-only without control group design. Jumlah sampel 32 ibu post partum diambil dengan teknik purposive sampling. Analisis bivariate yang digunakan ialah Two Way Anova. Hasil penelitian menunjukkan kecukupan ASI pada kelompok ekstrak daun kelor 15 (93,75%) sedangkan  kecukupan ASI pada kelompok ekstrak biji fenugreek 14 (87,5%). Tidak ada perbedaan yang signifikan antara ekstrak daun kelor dan ekstrak biji fenugreek terhadap kecukupan ASI pada ibu menyusui di wilayah kerja puskesmas linggang bigung, dengan nilai significancy pada hasil menunjukan (p=0.559>0.05)