Catfish farming offers strong potential for community economic growth. Catfish are resistant to various water conditions so that many people cultivate this fish. Just like other fish farming, the disease is the biggest obstacle in catfish farming. Changes in fish haematology in response to stress agents are indicators of the stress stage of fish, yielding useful information for curbing unfavourable conditions that can affect fish health. This study aimed to determine the haematological conditions of catfish in three different locations scattered in Tuban Regency as an indicator of fish health. The method used was descriptive by observing Erythrocytes, Leukocytes, Hemoglobin, and Hematocrit at 3 locations, namely in Tegalagung Village (a), Jenggolo Village (b), and Campurejo Village (c). The results of the erythrocyte at all locations were (a) 540,000 cells/mm3, (b) 1,980,000 cells/mm3, and (c) 1,690,000 cells/mm3. The results of the leukocyte count at all locations showed that (a) 301,000 cells/mm3, (b) 545,500 cells/mm3 and (c) 276,000 cells/mm3. Hemoglobin observations showed at the location (a) 16 g%, (b) 10.1 g% and (c) 10.8 g%. Hematocrit observation results showed at locations (a) 14%, (b) 13% and (c) 15%. Based on those results, it shows that catfish are in abnormal conditions. It is concluded that hematological parameters are outside the normal fish limits.

Koi (Cyprinus carpio) hatchery and aquaculture sector is highly beneficial as it is one of the Indonesian and international superior commodity. However, Koi are susceptible to pests and diseases. One of the diseases is Myxobolusis which is caused by the Myxobolus sp. parasite. The clinical symptom of Myxobolusis are lumps (boils) on the gills and cloudy reddish fluid secretion similar to pus. This research aims to determine the infection profile of Myxobolus through histology observation on gill tissue damage of koi cultivated in a ground pond. This research uses a descriptive method and a quantitative approach. This research observes the gill tissue damage of koi collected from the ground pond of Nglegok, Blitar Regency. The researcher observes the type of damage using a microscope with a magnification of 400x. This research uses a scoring method to assess the damage. Based on the result of histopathological observation, the infected fish exhibits oedema, haemorrhage, hyperplasia, lamellar fusion, and vacuolization. The percentage (%) of tissue damage in Myxobolus infected koi are 72%, 33%, 77%, 75% and 52% respectively. The average score shows moderate to severe damage levels. The observation result on water quality parameter (temperature, pH, DO, and CO2), as supporting parameter in water quality management, shows a tolerable condition and is not the primary cause of Myxobolus sp. infection. However, the transmission of infection may occur due to the presence of other organisms that act as Myxobolus sp. host.

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Viral Nervous Necrosis (VNN) is a Nodaviridae virus that attacks groupers, especially in the larval and seed stages. VNN attacks the brain organs, rapidly attacks the grouper fish receptors and then spreads to the brain via blood circulation. The mechanism of viral infection can occur because of the bond between the VNN adhesive and its receptor molecules in the grouper organ. The aim of this study was to compare the CD4 (Cluster Differentiation-4) values of VNN-infected groupers and healthy fish using immunohistochemical (IHC) analysis. The method used in this study was experimental exploratory by comparing and analyzing the response of CD4 cells in healthy groupers with groupers infected with VNN through IHC observations. The results of IHC test showed that the immunogenic epitope of 32.5 kDa VNN protein had the ability to induce tissue receptors of Humpback grouper (Cromileptes altivelis) by forming immune cells qualitatively. There are CD4 cells that are immune to specific tissue organs, i.e. eyes of the groupers, which are labeled with anti-CD4 secondary antibodies. It seems that immunogenic protein VNN with a molecular weight of 32.5 kDa has the ability to stimulate the expression of Humpback grouper immune cells such as CD4 in tissue organs, especially the eye organs qualitatively.