The hypothesis that changes in the IP amplitude of the fluorescence transient OJIP reflect changes in leaf photosystem I (PSI) content was tested using mineral-deficient sugar beet plants. Young sugar beet plants (Beta vulgaris) were grown hydroponically on nutrient solutions containing either 1 mM or no Mg(2+) and 2.1 µM to 1.88 mM SO(4)(2-) for 4 weeks. During this period two leaf pairs were followed: the already developed second leaf pair and the third leaf pair that was budding at the start of the treatment. The IP amplitude [ΔF(IP) (fluorescence amplitude of the I-to-P-rise) and its relative contribution to the fluorescence rise: ΔV(IP) (amplitude of the relative variable fluorescence of the I-to-P-rise = relative contribution of the I-to-P-rise to the OJIP-rise)] and the amplitude of the transmission change at 820 nm (difference between all plastocyanin and the primary electron donor of photosystems I oxidized and reduced, respectively) relative to the total transmission signal (ΔI(max) /I(tot)) were determined as a function of the treatment time. Correlating the transmission and the two fluorescence parameters yielded approximately linear relationships in both cases. For the least severely affected leaves the parameter ΔV(IP) correlated considerably better with ΔI(max) /I(tot) than ΔF(IP) indicating that it is the ratio PSII:PSI that counts. To show that the relationship also holds for other plants and treatments, data from salt- and drought-stressed plants of barley, chickpea and pea are shown. The relationship between ΔV(IP) and PSI content was confirmed by western blot analysis using an antibody against psaD. The good correlations between ΔI(max) /I(tot) and ΔF(IP) and ΔV(IP) , respectively, suggest that changes in the IP amplitude can be used as semi-quantitative indicators for (relative) changes in the PSI content of the leaf.
The main objective of this study was to evaluate the effects of salt stress on the photosynthetic electron transport chain using two chickpea lines (Cicer arietinum L.) differing in their salt stress tolerance at the germination stage (AKN 87 and AKN 290). Two weeks after sowing, seedlings were exposed to salt stress for 2 weeks and irrigated with 200 ml of 200 mM NaCl every 2 days. The polyphasic OJIP fluorescence transient and the 820-nm transmission kinetics (photosystem I) were used to evaluate the effects of salt stress on the functionality of the photosynthetic electron transport chain. It was observed that a signature for salt stress was a combination of a higher J step (V), a smaller IP amplitude, and little or no effect on the primary quantum yield of PSII (φ). We observed for AKN 290 a shorter leaf life cycle, which may represent a mechanism to cope with salt stress. For severely salt-stressed leaves, an inhibition of electron flow between the PQ pool and P700 was found. The data also suggest that the properties of electron flow beyond PSI are affected by salt stress.
Barley seedlings were pre-treated with 1 and 5 µM H 2 O 2 for 2 d and then supplied with water or 150 mM NaCl for 4 and 7 d. Exogenous H 2 O 2 alone had no effect on the proline, malondialdehyde (MDA) and H 2 O 2 contents, decreased catalase (CAT) activity and had no effect on peroxidase (POX) activity. Three new superoxide dismutase (SOD) isoenzymes appeared in the leaves as a result of 1 µM H 2 O 2 treatment. NaCl enhanced CAT and POX activity. SOD activity and isoenzyme patterns were changed due to H 2 O 2 pre-treatment, NaCl stress and leaf ageing. In pre-treated seedlings the rate of 14 CO 2 fixation was higher and MDA, H 2 O 2 and proline contents were lower in comparison to the seedlings subjected directly to NaCl stress. Cl -content in the leaves 4 and 7 d after NaCl supply increased considerably, but less in pre-treated plants. It was suggested that H 2 O 2 metabolism is involved as a signal in the processes of barley salt tolerance.
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