ABSTRAK: Buah kecombrang termasuk famili Zingiberaceae, dimanfaatkan sebagai rempah, flavor makanan, dan herbal tradisional. Penelitian ini bertujuan mengetahui karakteristik fisik dan identifikasi bioaktif fitokimia 3 klon buah kecombrang (warna ungu, merah, dan merah muda) secara kualitatif dan kuantitatif. Ekstrak diperoleh melalui maserasi serbuk selama 26 jam dengan 0.1% HCl (1:10) dalam methanol. Penelitian menunjukkan buah kecombrang merah muda memiliki karakteristik fisik dominan (panjang, diameter, keliling, berat, jumlah pipilan buah) dan warna tercerah. Sedangkan buah kecombrang ungu mengandung total fenolik, total flavonoid, dan aktivitas antioksidan tertinggi yaitu total fenolik 1,51±0,03 mg GAE/g ekstrak berat kering (bk), total flavonoid 0.38±0.03 mg QE/g ekstrak bk (ungu), aktivitas antioksidan 599.07±92.58 mg AAE/100 g (DPPH) dan 59.14±5.72 mg AAE/100 g ekstrak bk (FRAP). Analisis PCA (Principal Component Analysis) menunjukkan korelasi negatif antara karakteristik fisik dengan fitokimia dan aktivitas antioksidan.

ABSTRAKGonggong termasuk sejenis siput laut, biota endemik yang banyak hidup di pantai Pulau Bintan dan sekitarnya di Provinsi Kepulauan Riau. Gonggong merupakan ikon kota Tanjungpinang, Provinsi Kepulauan Riau. Sampai saat ini, penelitian gonggong masih sangat sedikit padahal siput ini merupakan spesies yang sangat potensial. Tujuan penelitian ini adalah untuk mengkarakterisasi protein histon dari siput gonggong Strombus sp. asal Bintan sebagai kandidat pangan fungsional. Karakterisasi profil protein menggunakan SDS-PAGE. Kadar protein pada gonggong dianalisis dengan menggunakan metode Bradford. Gonggong rebus bercangkang tebal diekstraksi dengan metode maserasi menggunakan pelarut etanol PA 95% dan uji aktivitas antimikroba menggunakan metode sumur. Asam amino dianalisis menggunakan HPLC. Hasil karakterisasi profil protein pada daging gonggong menunjukkan bahwa gonggong bercangkang tipis dan gonggong bercangkang tebal memiliki pita profil protein yang sama pada berat molekul 11-37 kDa, sedangkan profil protein pada lendir gonggong bercangkang tebal dan tipis memiliki pita protein yang sama pada berat molekul 37 kDa. Jenis protein pada spesies gonggong Bintan diprediksi merupakan protein histon karena hasil amplifikasi menggunakan primer protein histon H2A dan H2B didapatkan gen target pada 75 bp dan uji antimikroba pada bakteri S. aureus dan E. coli memiliki nilai DDH sebesar 25,65±0,02 mm dan 14,45±0,13 mm, sehingga diduga bahwa gonggong Bintan berpotensi sebagai kandidat pangan fungsional khas Kepulauan Riau. ABSTRACTGonggong is one of the sea snails, endemic species living on coastal waters of Bintan Island and surrounding islands of the Riau Islands Province. Sea snail gonggong is an icon of Tanjungpinang-Riau Islands Province. Until now, research this snail is the least, whereas it is potential species. The purpose of this study was to characterize histone protein from Bintan gonggong snail Strombus sp. as functional food candidate. Protein profiling used SDS-PAGE. Protein contents were analyzed by Bradford method. Boiled thick shelled gonggong were extracted by maseration method using ethanol PA 95% and antimicrobial activity tes using well method. Amino acid analized with HPLC. The result of characterization on protein profiles in meat gonggong showed that the thin-shelled and thick-shelled gonggong had the same band as protein profiles by 11-37 kDa and protein profiles in mucus gonggong were found the same band as protein profiles of 37 kDa. The type of protein in spesies Bintan gonggong had been predicted a histone protein because DNA identification using primer protein histone H2A and H2B had gen target of 75 bp. Antimicrobial activity test on S. aureus and E. coli bacteria had value DDH of 25.65±0.02 mm and 14.45±0.13 mm. In fact, gonggong snail was potentially as antimicrobial peptide, so it will make local functional food candidate from Riau Islands Province.

<p>Jahe merah<em> </em>(<em>Zingiber officinale </em>Roscoe var. Rubrum) merupakan tanaman rempah yang sering digunakan untuk pengobatan tradisional. Penelitian tentang senyawa bioaktif jahe merah telah banyak diteliti, namun penelitian untuk mengidentifikasi senyawa bioaktif sebagai sumber antioksidan menggunakan pendekatan metabolomik berbasis HPLC belum dilakukan. Tujuan dari penelitian ini adalah untuk mengidentifikasi komponen bioaktif jahe merah sebagai antioksidan dengan pendekatan metabolomik. Profil senyawa kimia dari ekstrak dan fraksi (heksana, kloroform, etil asetat, dan air) dianalisis menggunakan High Performance Liquid Chromatography (HPLC) yang dilanjutkan dengan analisis metabolomik <em>Orthogonal Projection to Latent Structure</em> (OPLS). Hasil uji metabolomik OPLS diperoleh fraksi etil asetat sebagai sampel yang paling berkontribusi terhadap aktivitas antioksidan. Sampel ini kemudian diidentifikasi menggunakan UHPLC-MS/MS dan diketahui bahwa komponen bioaktif atau <em>specific marker </em>pada jahe merah adalah senyawa [6]-gingerol. Kandungan senyawa [6]-gingerol merupakan senyawa yang paling dominan diikuti oleh 8-gingerol dan 10-gingerol dengan nilai masing-masing secara berurutan adalah 88,61±0,52; 23,59±0,41; dan 21,85±0,63 mg/g ekstrak.</p><p><strong>Identification of Active Components of Red Ginger (Zingiber officinale Roscoe var. Rubrum) as a Source of Antioxidants with HPLC-Based Metabolomics Approach.</strong> Red ginger (<em>Zingiber officinale </em>Roscoe var. Rubrum) is a rhizome used as a traditional medicine. Research on red ginger bioactive compounds has been widely studied but research to identify bioactive compound as source of antioxidant using HPLC-based metabolomic approaches has not been carried out. This study aims to identify the active compound of red ginger as an antioxidant with a metabolomic approach. Chemical profiles of the extracts and fractions (hexane, chloroform, ethyl acetate, and water) were analyzed using High Performance Liquid Chromatography (HPLC), followed by Orthogonal Projection to Latent Structure (OPLS) metabolomic analysis. The results of the OPLS metabolomic analysis showed that the ethyl acetate fraction is the sample that contributed the most to antioxidant activity. This sample was then identified using UHPLC-MS/MS showing that the bioactive component or specific marker in red ginger was [6]-gingerol. The [6]-gingerol content was the most dominant component, followed by [8]-gingerol and [10]-gingerol with values respectively 88.61±0.52, 23.59±0.41, and 21.85±0.63 mg/g extract.</p>

XYZ is a small industrial scale coconut chips producer who wants to develop their products and market. This study aimed to describe ideal characteristic of coconut chips, develop XYZ product and their marketing strategy. The research used survey method by distributing online questionnaires to respondents, and sensory tests (CATA, RATA, hedonic) by untrained panelists. As quantitative descriptive research, it was conducted in four stages: consumer surveys, mapping of sensory attributes of coconut chips by PCA, product improvement, and development of marketing strategies through STPD analysis and business model canvas (BMC). The results showed that the market opportunity for coconut chips was still available and prospective. Crispness, sweet taste, sweet aftertaste, and caramel aroma were ideal product characteristics of coconut chips after mapping of sensory attributes from three competitor products and XYZ product. XYZ product weakness was corrected for its sweet taste attribute by reformulation to increase sweetness, and the selected formula used a ratio of weight of sugar and coconut chips 2:8 (w:w). Efforts to increase consumer interest in product need to be carried out through marketing strategies targeting young people and the target market for the middle class, as well as a differentiating in the use of natural ingredients without preservatives and artificial coloring.