This research aims to determine the effect of the secondary metabolites of the fungi Simplicillium sp. on mortality, larval feeding capacity, larval growth, percentage of pupae and imagos, fecundity and fertility, secondary metabolites of the fungi Simplicillium sp. which effectively kills Spodoptera frugiperda. This research was conducted in the Plant Protection Laboratory of Agriculture Faculty, Jenderal Soedirman University, Purwokerto from October 2020 until January 2021. The method used in this research was a factorial randomized block (RBD) with two factors. The first factor is the concentration of secondary metabolite of the fungi Simplicillium sp. consisting of control (K0), 10% concentration (K1), 20% concentration (K2), 30% concentration (K3); and a synthetic insecticide with an active ingredient Emmamectin benzoate and Lufenuron 1 ml/l (K4). The second factor is the application method which consists of the spraying method on the larvae (A1) and the feed immersion method (A2). The observed parameters were mortality, feeding capacity, growth time, percentage of pupae and imagos formed, fecundity and fertility, and effective concentration. The result showed that: 1) secondary metabolite of the fungi Simplicillium sp. with a concentration of 20 percent larvae spraying method caused larvae mortality by 36.67 percent and the food immersion method caused larvae mortality by 45.00 percent, and decreased feeding activity by 26.12 percent; 2) secondary metabolite of the fungi Simplicillium with a concentration of 20 percent was able to inhibit the growth of larvae by 3.83 percent, pupae by 5,40 percent and imagos by 14.19 percent, reduced the number of pupae and imagos formed by 49.17 percent and 37.33 percent, and reduced fertility at a concentration of 10 percent by 11.30 percent; 3) secondary metabolite of the fungi Simplicillium sp. has not been effectively used as a control for S. frugiperda since it has an efficacy value of less than 80 percent.

Kelompok tani “Rukun Tani I” Desa Sumbang ,  Kecamatan  Sumbang Kabupaten  Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman hortikultur. Permasalahan utama yang dihadapi dalam peningkatan produksi bawang merah adalah tingginya serangan penyakit moler (Fusarium oxysporum) dan hama ulat bawang (Spodoptera exigua). Sosialisasi pengelolaan hama dan penyakit bawang  merah dengan menggunakan biopestisida Trichoderma sp. perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang hama dan penyakit bawang merah serta tindakan pengelolaannya dengan pestisida hayati berbasis Trichoderma.  Permasalahan mitra diatasi dengan beberapa pemecahan teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis hama dan penyakit bawang merah, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan aplikasi Trichoderma mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 %  dan 34,09 %. Aplikasi pestisida hayati  Trichoderma sp. pada petak demplot mampu meningkatkan pertumbuhan dan hasil bawang merah sebesar 7,09 % dan menurunkan intensitas hama dan penyakit  sebesar 34,16 %.

Weed pathogenic fungi play an important role in biological control of weeds. Weeds grow a lot among cultivated plants, the role of weed pathogenic fungi to cultivated plants is not yet known. This research aimed to determine the effect of weed pathogenic fungi on bok choy. The research was carried out in Tambaksogra Village, Sumbang District, Banyumas Regency at an altitude of 100 m above sea level. Randomized block design was used with treatments of weed pathogenic fungi (Fusarium sp., Curvularia sp., Chaetomium sp., Fusarium sp. + Curvularia sp., Fusarium sp. + Chaetomium sp., Curvularia sp. + Chaetomium sp., and Fusarium sp. + Curvularia sp. + Chaetomium sp.) repeated four times. The observed variables included symptoms, incubation period, disease intensity, disease incidence, infection rate, AUDPC, number of leaves, plant height, and plant fresh and dry weight. Results showed that Curvularia sp., Chaetomium sp., and Curvularia sp. + Chaetomium sp. did not affect on bok choy, while Fusarium sp., Fusarium sp. + Curvularia sp., Fusarium sp. + Chaetomium sp., and Fusarium sp. x Curvularia sp. + Chaetomium sp. caused disease intensity of 2.7, 2.7, 3.55, and 4.4%, respectively, and affected plant dry weight by 113.35, 82.36, 77, and 74.07 %, respectively, from control. Curvularia sp., Chaetomium sp., and Curvularia sp. + Chaetomium sp. could be used as a bioherbicide in bok choy cultivation.

Spodoptera frugiperda is a new pest in Indonesia that attacks corn and can cause up to 100 percent damageon young plants. As an invasive pest, information on potential indigenous natural enemies that cancontrol this pest is needed. This study aims to determine the effectiveness of indigenous entomopathogenic fungi and their application methods on mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. This study used a factorial Completely Randomized Block Design (RCBD) method consisting of 10 treatments. Treatmentstested werecombination of fungi species, namely Fusarium sp., Aspergillus oryzae, Entomophthora sp. with conidia density 109 ml-1, distilled water and cypermethrin at concentration of 1 ml.L-1 as a control. Two application techniques used were direct application on S. frugiperda larvae and diet test. Each treatment was repeated three times resulting in30 experimental units. Variables observed included mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. Results showed that the three fungi and cypermethrin treatment did not cause mortality, but reduced feeding activity, fecundity and fertility of S. frugiperda. Application of entomopathogenic fungi ondietswas more effective than directly spraying S. frugiperda larvae. The best treatment combination that suppressed feeding activity was the application of Aspergillus oryzae sprayed on S. frugiperda diet.It was also suspected that S. frugiperda larvae used in this test had developed resistance to cypermethrin.

Kesadaran masyarakat akan keamanan pangan dan gizi menyebabkan masyarakat  memberikan perhatian lebih besar pada kualitas dan keamanan produk sayuran yang dikonsumsi dan menginginkan makanan yang serba alami dan bebas dari bahan kimia, baik dari pestisida, hormon dan pupuk. Permasalahan mitra adalah  belum mengetahui paket teknologi budidaya sayuran secara ramah lingkungan, baik dari segi ekonomi, kesehatan, dan keamanan pangan yang dapat diterapkan untuk memanfaatkan pekarangan secara vertikultur aquaponik. Untuk mengatasi permasalahan mitra beberapa solusi teknologi yang ditawarkan adalah transfer teknologi melalui pendampingan, pendidikan, dan demplot.  Tujuan kegiatan ini adalah memanfaatkan lahan pekarangan di sekitar pemukiman dengan menanam tanaman sayuran ramah lingkungan dengan teknologi vertikultur aquaponik dan aplikasi basubio sebagai pengendali hayati dan pupuk hayati. Metode ceramah dan diskusi dilakukan sebagai media alih informasi yang bersifat interaktif dan berlangsung dua arah. Metode ini merupakan inisiasi program dengan harapan, kelompok mitra  mempunyai pengetahuan dasar yang baik tentang pengetahuan budidaya tanaman sayuran secara vertikultur aquaponik, memanfaatkan lahan kosong di sekitar rumah mereka, sehingga tercipta lingkungan yang nyaman, dan hasil panennya dapat dikonsumsi secara aman.  Penerapan program dilanjutkan dengan peningkatan ketrampilan anggota kelompok Dasawisma  melalui pelatihan dilengkapi dengan demplot. Hasil kegiatan yang dilakukan antara lain: budidaya sayuran secara vertikultur aquaponik yang ramah lingkungan. Pembuatan biofertilizer dan biopestisida berbahan aktif B. subtilis, pembuatan MOL (mikroorganisme lokal) dan pestisida nabati untuk mendukung kesehatan dan keamanan pangan.  Dampak dari kegiatan ini menambah pengetahuan dan ketrampilan kelompok Dasawisma mencapai 95% dan menambah indah lingkungan dan hasil sayurannya dapat dimanfaatkan dalam kebutuhan makan sehari-hari.