Abstrak Pendahuluan: Adenokarsinoma adalah jenis kanker prostat terbanyak didiagnosis sampai saat ini. Androgen receptor (AR) berperan penting dalam inisiasi, pertumbuhan dan progresifitas adenokarsinoma prostat, tetapi mekanisme AR masih belum jelas. Analisa ekspresi AR sebagai biomarker prognosis adenokarsinoma prostat belum pernah dilakukan di Sumatera Barat. Tujuan penelitian: Untuk menganalisa ekspresi tinggi AR dalam penentuan prognosis adenokarsinoma prostat di Sumatera Barat. Metode: Rancangan penelitian analitik observasional dengan desain cross sectional. Penelitian dilakukan bulan November 2019 sampai Oktober 2020. Sampel digunakan sebanyak 56 kasus adenokarsinoma prostat secara consecutive sampling yang tersimpan di laboratorium Patologi Anatomik di Sumatera Barat. Slaid yang telah terwarnai hematoxyline dan eosin (HE) dan blok parafin dikumpulkan. Selanjutnya dilakukan review slaid untuk menentukan Gleason score berdasarkan International Society of Urological Pathology (ISUP) 2014/WHO edisi 2016. Metode pewarnaan imunohistokimia (IHK) pada AR untuk menganalisis ekspresi protein secara semikuantitatif. Ekspresi tinggi AR jika interpretasi yang ditemukan strong. Analisis data secara statistik menggunakan uji Chi-square. Hasil: Hasil penelitian mendapatkan kasus adenokarsinoma prostat terbanyak adalah high grade Gleason score (Gleason score 8-10) yaitu 43 (76,79%) kasus dan ekspresi tinggi AR 29 (51,80%) kasus. Adenokarsinoma prostat dengan high grade Gleason score ditemukan ekspresi tinggi AR lebih banyak dibandingkan ekspresi rendah AR. Secara statistik terdapat hubungan yang signifikan antara ekspresi tinggi AR dengan Gleason score (p=0,018). Kesimpulan: Ekspresi tinggi AR merupakan penanda penting untuk progresifitas tumor. Ekspresi AR sebaiknya diperiksa pada kasus adenokarsinoma prostat untuk menentukan prognosis pasien.Kata kunci -- Adenokarsinoma prostat, Androgen receptor, Gleason score, Prognosis Abstract Introduction: Adenocarcinoma is the most type of prostate cancer diagnosed until now. Androgen receptor (AR) plays important roles in initiation, growth and progression of prostate adenocarcinoma. However, the mechanism of AR is unclear. Analysis of AR expression as the prognostic biomarker in prostate adenocarcinoma has never been done in West Sumatera. Aims: To analyze high AR expression in determining the prognosis of prostate adenocarcinoma in West Sumatera. Method: The observational analytic study was done with cross sectional design. This study was conducted from November 2019 to October 2020. Samples of 56 prostate adenocarcinoma were collected by consecutive sampling stored in Anatomical Pathology Laboratories in West Sumatera. Hematoxylin and eosin (HE) stained slides and paraffin blocks were retrieved. Slides were evaluated to review Gleason score based on the International Society of Urological Pathology (ISUP) 2014/WHO 2016. Immunohistochemistry (IHC) staining method with AR antibody was performed to discover protein expression semiquantitatively. High AR expression was characterized by strong interpretation. Statistical data analysis used Chi-square test. Results: The results showed that the most prostate adenocarcinoma was high grade Gleason score (Gleason score 8-10) in 43 (76.79%) cases and high AR expression in 29 (51.79%) cases. High grade Gleason score of prostate adenocarcinoma showed high AR expression more than low AR expression. There was statistically significant correlation between AR expression and Gleason score (p=0.018). Conclusion: High AR expression is the important marker of tumor progression. We suggest that AR expression should be performed in patients of prostate adenocarcinoma for prognosis. Keywords -- Prostate adenocarcinoma, Androgen receptor, Gleason score, Prognosis
Appendicitis is a common cause of abdominal pain that requires emergency surgery in each age group and sex. The incidence of appendicitis in the world reaches 3442 million cases each year. The survey at 15 provinces of Indonesia in 2014 showed 4,351 cases of hospitalized appendicitis.This aims to obtain information about the profile of patients suffering from appendicitis at the Siti Rahmah Islamic Hospital Padang West Sumatra in the period of 2016 – 2017.The study is descriptive by using secondary data from patient’s medical record.This study was conducted from January to February 2019, the sample are 65 samples by simple random sampling technique.The results showed that the highest age group of incidences was in the young adults (17-45 years), which is 39 people (60%), and the highest sex group was male 36 people (55.4%), leukocytosis with 50 people (76.9%), classification was acute appendicitis with 42 people (64.6%), pain onset was 24-48 hours 33 people (50.8%). The highest age group of 42 patients with acute diagnosis was young adults (73.8%), four patients with the chronic diagnosis were highest at young adults group, and 19 patients (36.8%) with the diagnosis of perforation were highest at the older adults group.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.