POLD1 (DNA polymerase delta 1, catalytic subunit) is a protein-coding gene that encodes the large catalytic subunit (POLD1/p125) of the DNA polymerase delta (Polδ) complex. The consequence of missense or nonsynonymous SNPs (nsSNPs), which occur in the coding region of a specific gene, is the replacement of single amino acid. It may also change the structure, stability, and/or functions of the protein. Mutation in the POLD1 gene is associated with autosomal dominant predisposition to colonic adenomatous polyps, colon cancer, endometrial cancer (EDMC), breast cancer, and brain tumors. These de novo mutations in the POLD1 gene also result in autosomal dominant MDPL syndrome (mandibular hypoplasia, deafness, progeroid features, and lipodystrophy). In this study, genetic variations of POLD1 which may affect the structure and/or function were analyzed using different types of bioinformatics tools. A total of 17038 nsSNPs for POLD1 were collected from the NCBI database, among which 1317 were missense variants. Out of all missense nsSNPs, 28 were found to be deleterious functionally and structurally. Among these deleterious nsSNPs, 23 showed a conservation scale of >5, 2 were predicted to be associated with binding site formation, and one acted as a posttranslational modification site. All of them were involved in coil, extracellular structures, or helix formation, and some cause the change in size, charge, and hydrophobicity.

An experiment was undertaken to elucidate the genetic relationship between different quantitative traits for commercial cultivation and to evaluate selection criteria in pea breeding programs in five inbred parents. Their 17 F4’s derivatives in pea (Pisum sativum L.) evaluated ten characters during the winter season (November to February) of 2017-18 at the research farm, BSMRAU, Gazipur, Bangladesh. Analysis of variance explored significant differences among the genotypes for all the characters.  Phenotypic coefficients of variation (PCV) were close to genotypic coefficients of variation (GCV) for all the characters indicating less influence on the environment and potentiality of selection. A high heritability relationship with high genetic advance was observed for plant height, pod per plant, hundred seed weight, and seed yield per plot. Pod length showed a highly significant positive correlation with pod width and hundred seeds weight. Only days to first flowering showed a highly negative correlation with pod length and hundred seed weight. Path coefficient analysis revealed that plant height, pod per plant, and seeds per pod had a highly positive effect on yield per plant. Therefore, associating and selecting those traits, yield improvement must be possible in pea, and the days to maturity, plant height, pods per plant, pod length, and seed showed a considerable positive and highly significant correlation with plant height, pod per plant, seed per pod, and yield per plant at both genotypic and phenotypic levels indicating yield could be increased with the increase of days to maturity, plant height, pods per plant, pod length, and seed.

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Pea (Pisum sativum L.) is the most important grain legume crop grown worldwide and used as food and fodder. An experiment was undertaken to elucidate the genetic variability in five inbred parents and their 17 F4’s derivatives in pea evaluating ten characters during the winter season of 2017-2018 at the research farm, BSMRAU, Gazipur, Bangladesh. Analysis of variance explored significant differences among the genotypes for all the characters. Phenotypic coefficients of variation were close to genotypic coefficients of variation for all the characters indicating less influence on the environment and potentiality of selection. High heritability associated with high genetic advance was observed for plant height, pod per plant, hundred seed weight, and seed yield per plot. Days to 50% flowering and days to first flowering showed a highly significant positive correlation at both genotypic and phenotypic levels. Plant height showed a highly significant positive correlation with pods per plant, seeds per pod, and seed yield per plant. Pod length showed a highly significant positive correlation with pod width and hundred seeds weight. Pods per plant, pod width, and seeds per pod showed a highly positive correlation with seed yield per plant and 100-seed weight. Only days to first flowering showed a highly negative correlation with pod length and hundred seed weight. Path coefficient analysis revealed that plant height, pod per plant, and seeds per pod had a highly positive effect on yield per plant. Therefore, the yield improvement of pea is plausibly possible by associating and selecting those plant traits. Furthermore, two crosses such as Zhikargacha×IPSA Motorshuti 1 and Zhikargacha×IPSA Motorshuti 2 may be chosen for further breeding programs. SAARC J. Agric., 20(1): 29-39 (2022)