Abstract. Learning achievement is an achievement of a student in the academic field for the results of his hard work during the learning process. There are several factors that affect learning achievement, including interest and motivation to learn. The purpose of this study is to analyze the effect of learning interest and learning motivation on academic achievement. This research was conducted with an analytic observational design using a cross-sectional study. The subjects for this research were calculated by using estimating proportion formula. There were 69 subjects who met the inclusion and exclusion criteria and were selected by simple random sampling technique. Data was taken by distributing study interest questionnaires to measure interest in learning and academic motivation scale questionnaire to measure learning motivation while learning achievement data was obtained from the latest GPA scores. Data was analyzed by using multiple regression, t test, f test, determination test, and partial influence test to assess the effect of interest and learning motivation on learning achievement of freshman students of the Faculty of Medicine, Bandung Islamic University batch 2021. The results shows that there is a significant influence between learning interest and learning motivation on learning achievement. In the determination test, it is found that interest in learning has an effect 7.9% and learning motivation has an effect of 37% to learning achievement. With a total effect on determination test 44.9% to learning achievement and 51.9% is a factor that is not analyzed in this study. Abstrak. Prestasi belajar merupakan sebuah capaian seorang siswa dalam bidang akademik atas hasil kerja kerasnya selama proses pembelajaran. Terdapat beberapa faktor yang mempengaruhi prestasi belajar antara lain adalah minat dan motivasi belajar. Tujuan penelitian ini yaitu untuk menganalisis pengaruh minat dan motivasi belajar terhadap prestasi belajar. Penelitian ini dilakukan dengan desain observasional analitik dengan menggunakan studi cross-sectional. Subyek penelitian dihitung menggunakan rumus estimasi proporsi sebuah populasi. Subyek penelitian berjumlah 69 orang yang memenuhi kriteria inklusi dan ekslusi serta dipilih dengan tehnik simple random sampling. Data diambil dengan menyebarkan kuesioner study interest questionnaire untuk mengukur minat belajar dan kuisioner academic motivation scale untuk mengukur motivasi belajar sedangkan data prestasi belajar diperoleh dari nilai IPK terakhir. Data dianalisis menggunakan regresi berganda, uji t, uji f, uji determinasi dan uji pengaruh parsial untuk menilai pengaruh minat dan motivasi belajar terhadap prestasi belajar mahasiswa tingkat I Fakultas Kedokteran Universitas Islam Bandung angkatan 2021. Pada uji pengaruh parsial ditemukan minat belajar memberikan pengaruh sebesar 7,9 % dan motivasi belajar memberikan pengaruh 37 % terhadap prestasi belajar. Dengan total pengaruh pada uji determinasi kedua variabel terhadap prestasi belajar sebesar 44,9% dan 51,9% merupakah faktor yang tidak dianalisis pada penelitian ini.