The experiment was conducted at Lath house / Department of Horticulture and Landscape at the College of Agriculture / Tikrit University for the period from 17/3/2020 until 2/9/2020 on Catharanthus Roseus (L.) G. Don plant variety Pacifica X P Mix. The experiment was carried out using two factors under with Randomized Complete Block Design (R.C.B.D) in split plot, arranged into two factors which were put into the main plots involved applying water tension to the plant in three levels of field capacity (100%, 75% and 50%). The second factor which was placed in the sub plots, was sprayed on the shoots until completed wetness with four concentrations (0, 100, 200 and 300)mg.L−1. The results showed the superiority of (75%) field capacity and significant difference was recorded on most of the studied traits, where the leaf content characteristic of Vinblastine was recorded (114.750) mcg.−1. The root content characteristic of Vinblastine alkaloid was recorded (27.56) mcg.−1, and the root content characteristic of Vincristine (22.88) ) mcg.1 1was recorded, compared with the two water stress treatments (100% and 50%) Field capacity. Whereas, level of (100%) field capacity, outperformed the leaf content of Vincristine, which recorded (86.15) mcg.−1, which did not significantly -differentiate with the water tension treatment A2 at a level (75%) field capacity, which recorded (84.69) mcg.−1. As for the treatment of spraying with (Proline), the results showed that the treatment of B4 was superior to a concentration of 300 mg.L−1 in all studied traits (Vinblastine and Vincristine leaf and root content) compared with all concentrations.