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The paper shows the effectiveness of methods of somaclonal variability in combination with multiple individual selection for creating new soybean varieties characterized by early ripeness and increased seed productivity for sub-arid regions of Russia and Kazakhstan with cold continental climate. Soybean breeding material was created using biotechnology methods combined with multiple individual selection. Higher regeneration potential of soybean tissues of SibNIIK 315 variety compared to Omskaya-4 variety was revealed. The ability to regenerate is associated with the adaptability of SibNIIK 315 variety, which is confi rmed by the wider area of its cultivation in various ecological and geographical conditions in fi ve regions of Russia and Kazakhstan. Tissues of apexes and cotyledonary nodes of R21 somaclonal line differed from the original SibNIIK 315 variety by the increased activity of callus formation, regeneration and growth of shoots. Tissues of regenerated plants passed repeatedly through the regeneration cycle according to the protocol of recurrent regeneration. Due to this, high morphogenic activity of R21 tissues can be the result of autoselection in vitro. The analysis of phenotypic variations in early generations of soybean somaclones showed an asymmetric distribution of deviations by the main breeding and marker traits: duration of vegetation, plant height and seed productivity, as well as dependence of distribution on weather conditions. In a dry year most somaclonal lines had a higher seed productivity, whereas in favourable conditions of a humid summer only every third line was superior to the original variety. This indicates increased levels of ontogenetic adaptation of somaclones in extreme drought conditions.

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The search for new raw materials of biologically active substances of natural origin is an urgent task for the modern period. Peat in this respect is a relatively cheap and almost unlimited raw material base. Peat of swamps can be used widely in agriculture for receipt of biologically active substances are of great interest in the territory of the Gorny Altai. The purpose of this work is to study the composition of organic matter of peats of the Gorny Altai, to choose peat raw materials for biologically active production and to study their biological activity. The object of the study was 46 swamps of the Gorny Altai. The following analyses were carried out in peats: botanical composition, degree of decomposition, ash content, group composition of peat organic matter. The composition of humic acids (ha) was analyzed by IR spectroscopy. For determine of the biological activity of humic acids was used plant tissue cultured . The results of the research allowed to distinguish peats by the content of HA: buckbean peat (47.0% of HA), wood peat (50.0% of HA), fern peat (55.0% of HA), grass peat (30.0–45.0% of HA), sedge peat (5.6–58.0% of HA), grass-buckbean peat (43.0–56.5% of HA) and to outline the raw material base for the production of BAS – peatland Turochak. According to the optimal characteristics of HAs, a sample was taken from a depth of 325-375 cm, HA was isolated. The biological activity In humic acid was determined with the use of plant tissue culture. High biological activity are proven of the preparations of HA from the peatland Turochak, which resulted in the acceleration of microclonal propagation of plants in vitro.

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