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SUMMARY : Twenty strains of aerobic, facultatively anaerobic, chitinoclastic bacteria have been isolated from marine mud by enrichment cultures. Each was able to derive its full carbon and nitrogen requirements from chitin. None was an obligate chitinovor.These cultures comprised four new species of Achromobacter, two new species of Pseudomas, one new species of Flavobacterium and one new species of Micrococcus. Detailed descriptions for each species are appended.Each organism was able to liberate ammonia and reducing sugar from the chitin molecule, Glucosamine and acetic acid were not detected in the cultures, possibly because of their ready availability as supplementary nutrients.

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One of the more difficult and important problems encountered in analytical work on bacterial spores is the removal of the nonsporulating and unlysed vegetative cells found in all cultures which have attained maximum sporulation. A number of methods reported in the literature which have given satisfactory removal of vegetative cells of a number of species gave no appreciable separation of the spores and cells of Bacillus stearothermophilus NCA numbers 1518 and 7900, or Bacillus cereus. Reported methods which were here unsuccessful included attempted destruction of the vegetative cells by alteration of the osmotic environment, autolysis in distilled water (Church et al.,