1. Gentamicin was injected subcutaneously and intramuscularly into 5 groups of 10 laying hens and its concentration was determined in albumen, yolk and whole egg. 2. Groups 1 and 3 were intramuscularly injected with doses of 10 and 25 mg/kg while groups 2, 4 and 5 were subcutaneously injected with doses of 10, 25 and 50 mg/kg, respectively. 3. The final gentamicin concentration in albumen was measured on d 3 for groups 1 and 2; on d 4 for groups 3 and 4, and on d 5 for group 5. Concentrations in yolk and whole egg were measured on d 7, 10 and 12. 4. Gentamicin recovery was as follows: 2% in groups 1 and 2, 2.5% in groups 3 and 4, and 3% in group 5. 5. Most of the residue (approximately 90%) was recovered from the yolk.
The objective of this study was to determine the effects of different pretreatment agents such as chitosan (0.5% (w/v), pH 2.9-3.2) and lactic acid (0.5% (v/v), pH 2.5-2.7) on the chemical and sensory qualities of mussels stored at 4ºC. Mussels were dipped in 100 mL of 0.5% solution of lactic acid (v/v) and chitosan (w/v) at room temperature (22ºC) for 15 min. Mussels from the control group were dipped in 100 mL of sterile distiled water (2% NaCl) without chitosan and lactic acid. Treatment of mussels with lactic acid and chitosan at the beginning of the experiment (day 0) for 15 min reduced bacterial counts of total aerobic mesophilic bacteria (0.53-1.07 log) psychrotrophic bacteria (0.11-0.13 log), Lactobacillus spp. (0.46-1.30 log), Enterobacteriaceae (0.43-0.48 log) and coliform bacteria (0.52-0.66 log). Total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), trimethylamine nitrogen (TMA-N) and histamine values of control group mussels were increased from (day 0) 13.1 mg N 100 g , 4.86 mg N 100 g -1 and 7.75 ppm at the end of the storage period (day 11), respectively (P<0.05). The results indicated that shelf-life of mussels stored at 4ºC were limited to 4 days in the control group. However, mussels pretreated with lactic acid and chitosan were stored for 6-7 days and the shelf-life of mussels was extended for ca. 2-3 days, as compared with the control group (P<0.05).
The aim of the study is to evaluate the precence of Hepatitis A virus (HAV) and enteroviruses (EV) in milk samples. Raw milk and whey samples (n=50), collected from eight different open air markets in Samsun (Turkey) were analysed for HAV and enteroviruses by RT-PCR. Viral RNA was extracted using the rapid acid guanidinium thiocyanatephenol- chloroform method. HAV primers were selected from sequences of the VP2 and VP4 capsid region. Enterovirus primers were selected from the 5' non-translated region, which is the most conserved region in an enterovirus genome. Four samples (8%) raw milk were positive for EV RNA. However, HAV RNA was not determined the milk samples. This is the first report on the detection of HAV and EV RNA in cow milk samples in Turkey. This study highlights the interest for contamination by HAV and EV in milk samples from different areas
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