Annotation. P. aeruginosa, A. baumannii are the causative agents of severe wound infections, ventilator-associated pneumonia, catheter-associated septic conditions and other severe pathological processes. The ability of bacteria to form biofilms in affected tissues plays an important role in the pathogenesis of these diseases. Therefore, the purpose of our work is to study the influence of certain external factors on the ability of bacteria to form biofilms. The ability of bacteria to form biofilms in affected tissues plays an important role in the pathogenesis of these diseases. 5 clinical strains of P. aeruginosa and 5 strains of A. baumannii were used in this research. The ability to form biofilms was studied in 96-well polystyrene plates by the spectrophotometric method, which is based on the ability of the crystal violet dye to bind to the cells and matrix of the biofilm. Statistical processing of the obtained results was carried out using Excel Microsoft Office tables. The effect of the presence of certain sugars and nitrogen-containing compounds in the cultivation medium on the processes of biofilm formation by pseudomonads and acinetobacteria was investigated. As a result of the research, it was established that acinetobacteria show the ability to form films, unlike pseudomonads, under the condition of their cultivation in sterile distilled water. The introduction of certain carbohydrates or urea into the cultivation medium intensified the biofilm-forming properties of pseudomonads to a much greater extent, compared to acinetobacteria. According to the research results, it was established that the most active process of biofilm formation occurs in both types of non-fermenting Gram-negative bacteria when there are animal blood serum proteins in the culture medium. The formation of biofilms is one of the main strategies that increases the survival of bacteria in the environment, as well as in the human body. The ability of bacteria to form biofilms is, among other things, a factor in the pathogenicity and virulence of bacteria. So, in recent years, special attention has been paid to the ability of microorganisms to form biofilms, and to the study of factors affecting this process.

Annotation. The use of artificial lung ventilation in patients of intensive care unit often leads to airway contamination of conditionally pathogenic microorganisms and the development of ventilator-associated pneumonia. The clinical manifestations of ventilator-associated pneumonia can vary widely from mild to development of critical conditions accompanied by septic shock. This significantly worsens the patient's condition and prognosis for effective treatment. The paper presents the results of the study of the use of adhesive antiseptic compositions based on the domestic antiseptic drug decamethoxin in order to give antimicrobial properties to intubation tubes. An antibacterial adhesive was applied to the surface of segments of the endotracheal intubation tube, after which the samples were dried in a sterile box. The sensitivity of the microorganisms to the components of the antiseptic compositions was studied by the diameter of the growth retardation zone around the studied fragments of endotracheal intubation tubes placed in the thickness of the pre-seeded microorganism’s dense nutrient medium. The results were statistically analyzed using standard application package for biomedical research “STATISTICA 5.5”. It has been proved that adhesive film-forming compositions based on decamethoxin confer pronounced antimicrobial properties to endotracheal intubation tubes. The most susceptible to antimicrobial activity were gram-positive microorganisms of the genus Staphylococcus, and the most resistant were representatives of gram-negative bacteria of the genus Acinetobacter. The obtained research results allow us to predict the effectiveness of the use of adhesive hydrophilic and hydrophobic antiseptic compositions based on the domestic antiseptic decamethoxin to provide protective properties of the surface of endotracheal tubes, prevent the process of bacterial film formation and prevention of ventilator-associated patients.