Knowledge about the distribution of living organisms on Earth is very important for many areas of biological science and understanding of the surrounding world. However, much of the existing distributional data are scattered throughout a multitude of sources, such as taxonomic publications, checklists and natural history collections and often, bringing them together is difficult. A very successful attempt to solve this problem is the GBIF project, which allows a huge number of researchers to publish data in one place in a single standard. Our dataset represents a significant addition to the occurrences of amphibians in the Volga, Don riverine basins and adjacent territories. The dataset contains up-to-date information on amphibian occurrences in the Volga river basin and adjacent territories, located for the most part on the Russian plain of European Russia. The dataset is based on our own studies that were conducted in the years 1996-2020. The dataset consists of 5,030 incident records, all linked to geographical coordinates. A total of 13 amphibian species belonging to nine genera and six families have been registered within the studied territory, although the distribution of amphibian species in this region of Russia has not yet been fully studied. This is especially relevant with the spread of cryptic species that can only be identified using molecular genetic research methods. The main purpose of publishing a database is to make our data available in the global biodiversity system to a wide range of users. The data can be used by researchers, as well as helping the authorities to manage their territory more efficiently. All occurrences are published in GBIF for the first time. Most of the data are stored in field diaries and we would like to make it available to everyone by adding it in the global biodiversity database (GBIF).

The influence of unique natural conditions in the Sarykum sand dune on biochemical and tech nological properties of grapes has been studied, as well as the taxonomic composition of yeast living on it. The occurrence of active forms of saccharomyces being of interest to biotechnology has been shown. The biolog ical role played by a major component of sabulous soil, i.e., silicon dioxide, has been analyzed.

Goal. To study the toxicity of the modern variety of insecticides from different classes of chemical substances at the level of the mean concentration (СК50, %) of the active substance for the common cereal aphids, cabbage flea, rape blossom weevil, common spider mite. Based on these data to determine the rate of resistance of natural populations of these species of phytophagous arthropods to insecticides. Methodology. The sensitivity of phytophagous arthropods was studied on natural populations collected on crops of winter wheat, canola, soybeans in the Kiev region in 2015—2018. For the poisoning of cereal aphids and mites using the method of immersion of the populated lists wheat and soybeans, and flea beetles and pollen beetle — dip them in a gauze bag for 3 seconds in the appropriate solution of insecticides. Were taken 24 hours after poisoning. Toxicological parameters were calculated by using Proban. Results. Identifies the different sensitivity of the natural arthropod populations to current insecticides. In terms of the mean concentration (СК50, %) of the active substance rapeseed pollen beetle were most sensitive to Configure, while the rest of the drugs is almost the same. Over the years research is more resistant to Decis Profi turned ordinary spider mites. This pest was a higher resistance. Conclusions. The sensitivity of arthropods to insecticides of different chemical classes depends on the characteristics of the pest and the properties of the drug. Indicators of resistance are determined by the biology of the phytophage and terms of use of insecticide. The highest PR was observed for polyvoltine species (aphids, mites). Low levels of resistance to phytophagous pyrethroid insecticides can be explained by two factors: a smaller volume of use or reversion (return of chuvstvitelnosti to the initial level).

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Goal. To substantiate and develop an anti-resistant system of protection of crops and perennials from harmful arthropods. Methods. The sensitivity of phytophages on natural populations collected on winter wheat, soybean, and rapeseed crops was determined by various methods of their poisoning: immersion of insects on inhabited plants, immersion of insects in gauze bags for 3 seconds. in a suitable solution of insecticide. The methods are based on the study of the pest’s response to a series of doses of the drug — from the minimum, causing mortality of 5—10% of normal-sensitive individuals, to the maximum (mortality of 90% or more). Toxicological parameters were calculated using the PROBAN program. Results. Different sensitivity of natural populations of arthropods to modern insecticides is revealed. Techniques for the rational use of insecticides populations and the use of alternative biological and immunological methods. Conclusions. An effective measure to prevent and inhibit the formation of resistant populations of harmful arthropod species to insecticides is an anti-resistant plant protection system. It includes monitoring the resistance of natural populations of harmful phytophagous species, identifying the type of resistance and the rational use of chemicals.