Introduction. The integration of computer technologies into various fields of science allows the development of new methodologies, hybrid information systems with advanced capabilities, such as EcoIS bioinformation system for monitoring the environment with the use of biological data detectors.Problem Statement. The development of innovation bioinformation systems with biological data detectors is a very important task, as they have numerous advantages: allow rapid diagnostics and testing of chemicals in thefirst moments of their action, may be incorporated easily into electronic registration systems, may serve as elementary analytical units with data coding capabilities, etc.Purpose. The purpose of this research is to make a comprehensive analysis of different types of biological data detectors to develop a physical model of a biosensor capable of encoding signals and a bioinformation system with such detectors.Materials and Methods. The comparative analysis of information systems with functions of ecomonitoring and different types of biosensors have been used; the data are taken from electrophysiological experiments on registration of chemosensitive transmembrane electric currents in voltage clamp and patch clamp modes.Results. The physical model of biosensor has been developed and tested. The integration of the developed biosensors into the electronic bioinformation system by the example of EcoIS authors’ system has been demonstrated. Neuron-like biosensor has been considered an abstraction in the unity of its functions: signal receiver — filter — analyzer — encoder/decoder, where the input information is obtained in the form of chemical structures or electrical signals, after the conversion (recoding) of information it is registered as electrical signals with changed characteristics. The prospects for developing the cutting-edge methods for information protection in systems with biosensors have been shown. Conclusions. This development may be used for creating a bioinformation system for environmental moni toring with integrated biosensor system and data protection based on the principles and achievements of contemporary biophysics.

Background. In present publication we generalized and analyzed deeply the experience of some biosensors studying in biophysical experiments with aim to incorporate them further to electronic information systems. Output biosensor electrical signals were input ones to electronic information system making their connection into joined bioinformation system. Materials and methods. Methods of comparative analysis of the characteristics of input and output electrical information signals of biosensor were applied; its physical and mathematical models were developed. For biosensor properties studies the methods of transmembrane electric currents recording in voltage-clamp mode as well as patch-clamp on hippocampal neuronal membranes were used. Results. Biosensor concept and their general characteristic were given, corresponding prototypes were observed. The physical model of biosensor was developed and some test results of this device were suggested. The biosensor was examined as abstraction in consistent unity of its functions: signal receiver — filter — analyzer — encoder/decoder. A brief mathematical description of biosensor functioning was given as well as corresponding algorithm. As a result of performed works the possibilities of this biosensor incorporation to bioinformation electronic systems were substantiated and the example of such system «EcoIS» was observed. Conclusion. In conclusion following results of the works were summarized. The detailed description of technical devices — biosensors as elements of biomedical information systems were done as well as analysis of electrical information signals at output of biosensor, its ability to encode information and detailed analysis of the possibility to incorporate this biotechnical device into electronic information systems due to biosensor output electricals signals.

Objective: To investigate the possible mechanisms of splenozide influence on oxidative stress and metabolic disorders in tissues caused by acute nitrite intoxication in rats. Materials and Methods: Nitrite poisoning (NaNO 2 , 60 mg/kg, s.c.) was induced in rats pretreated with splenozide (natural metabolite, nucleoside complex; 3 mg/kg, i.p.). The parameters of lipid peroxidation (LPO), antioxidant defense system (superoxide dismutase [SOD], catalase [CAT], glutathione content [GSH], glutathione reductase [GR], glutathione peroxidase [GPx], glucose-6-phosphate dehydrogenase [G6PDH]), lactate and pyruvate concentrations, NAD + /NADH ratio in cytosol and mitochondrial succinate dehydrogenase (SDH) activity were evaluated in the liver, myocardium and brain. Results: Splenozide pretreatment decreased LPO, stimulated the G6PDH, GR and GPx activity, and increased the intracellular glutathione level in all studied tissues. Its effect Department of Hypoxic States, on SOD, CAT and SDH activity depended on the type of tissue studied. Splenozide caused a reduction of lactate concentration and accumulation of oxidized NAD in cytosol. Conclusion: Splenozide demonstrated a weak scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals in vitro. Its antioxidant effect during nitrite intoxication may be due to the maintenance of glutathione recycling activity through the activation of NADPH-dependent reactions, redox state restoration and antiacidotic effect.

Purpose. The purpose of the work was to demonstrate the applications of methods of database construction on the example of information about rainbow trout and viral infections affecting it. In process of such databases construction for electronic information systems it is necessary to find the ways of biological data preparation for each of solved tasks, than to make an adequate processing of these input data. Further step is the use of the methods of object-oriented system analysis for the aforementioned database construction in optimal way.Methodology. The methods of object-oriented system analysis, ER-diagram design, and the methods of computer databases construction were used in process of present work fulfillment.Findings. At the initial stage of the work some fish databases known in the world were observed. The peculiarities of biological objects (fishes) that have to be taken into account for this task fulfillment were analyzed. Further the approach of object-oriented analysis for constructing of computer databases in optimal manner was suggested. The first logical steps of algorithm for construction of databases with relative information about fish were described as well the practical recommendations for the development of databases with information concerning domestic biological organisms (on example of rainbow trout, its viral infection) for electronic information systems were done.Originality. No large-scale implementation of contemporary information-computer technologies in Ukraine was done yet. The obtained results would be contributed to further intensive implementation of contemporary information technologies for the development of domestic fishery industry.Practical value. Rainbow trout is important specie for fishery economy; its studying as well as viral infections affecting it are of great value for food safety. Information computer technologies application suggested in the work would make this branch of economy more effective in Ukraine and in the whole world.