Aim. The search for ways to create hepatoprotective food additives based on biologically active substances of plant origin and bee honey is an important area of research in the field of biology and medicine. Materials and Methods. The material for the research was bees of Ukrainian breed (Apis mellifera sossimai). The process of enriching the natural properties of natural honey was carried out in a natural way through the bee family by feeding colonies of bees with a special composition of natural origin components based on Avéna satíva and Silibum marianum. Sieve honey with monofloral honey from white acacia (Robinia pseudoacacia) 55.0–55.0% was used for feeding of the bee families. The first research group was exclusively fed honey water (control). For the second and third experimental groups, Avena Sativa and Milk Thistle Dry Extract were introduced into the main feed, respectively. Preparation of honey water was carried out on distilled water. Each group of bee families was evaluated according to the complex of biological and economic-useful features during the whole study period. The concentrations of active substances in the obtained batches of honey were determined. Ferulic acid was chosen as the active substance for oat, sum of Silibinin A and Silibinin B for milk thistle. The mass concentration of ferulic acid was measured according to European Standards EN 15662:2008 using Gas Chromatography-Tandem Mass Spectrometry following acetonitrile extraction/partitioning and clean-up by dispersive solid phase extraction (Quechers) method. The limits of quantitation of ferulic acid and sum of Silibinin A and Silibinin B were 0.02 mg/kg. The high content (83.3±0.4 mg/kg) of Silibinin flavonoids (A and B), Isosilibin (A and B), Silichristin A, etc. in honey persists. Instead, ferulic acid precipitated in fairly low concentrations of honey (1.13±0.02 mg/kg). Conclusions. The obtained results indicate that the extract of milk thistle is promising for the creation of enriched natural honey with hepatoprotective properties, as the biologically active components of this plant have characteristics that allow to obtain the final product with a high content of active substances. Key Words: honeybee, biological active substances, hepatoprotectors.

ABSTRACT. In the context of increasing anthropogenic load, the ability of the body to maintain high-level resistance to various harmful factors is essential. Thus, the creation of effective tools that can increase the level of adaptive capacity of the human body under conditions of the environmental hazards is a topical issue of modern medicine. Objective. Scientific substantiation of the technology of creation of enriched natural honey containing bioactive substances — adaptogens, capable to increase adaptive capabilities of the body under conditions of environmental hazards. Materials and Methods. The material for this study were Ukrainian breed bees. The process of enriching the natural properties of natural honey was carried out in a natural way through the bee family, by feeding colonies of bees with a special composition of natural origin components based on common oat (Avtina satHva) and milk thistle (Silybum marianum). Results. The analysis of the regulatory framework governing the use of honey with phytoadditives and bee products in Ukraine has proved that currently there is a lack of scientifically grounded technology of natural honey enrichment, which is realized through the bee family by feeding optimally selected qualitative and quantitative composition of high-quality components of natural origin. The authors proposed a model of toxicological and hygienic evaluation of raw materials and finished product based on enriched natural honey, which allows carrying out toxicological support of the process of creating an innovative product — enriched natural honey, and developing a modern technological standard of the finished product according to the evaluation results. Study results have proved that the most promising medicinal raw material for the creation of enriched natural honey is milk thistle (Silybum marianum) extract. Conclusion. The results of the first stage of the study confirm the prospect of further research on natural adaptogens for the development of innovative products based on natural honey and bioactive substances of plant origin, which will allow obtaining new useful varieties of honey and significantly improving the economic performance of enterprises producing these products. Key Words: biotechnologies, honey, bioactive substances, adaptogens.