Aim. To conduct a morphological study of the renal glomerulus in experimental diabetes mellitus and to study the pathomorphological features of the development of diabetic nephropathy. Methods. The study was carried out on 25 male Wistar rats. Modeling of diabetes mellitus was carried out by intraperitoneal injection of streptozotocin at a dose of 65 mg/kg. For more selective modeling of type 2 diabetes, the rats of the control group were intraperitoneally administered a solution of cytoflavin, calculated by the dosage of nicotinamide 115 mg/kg. In the comparison group 1 ml of physiological solution was administered in a similar manner. On day 28 of the experiment, the animals were euthanized under ether anesthesia, the kidneys were extracted, purified and washed with physiological saline. The material was fixed in a 10% neutral formalin solution. Using the morphometric method, the area of the renal glomeruli and the area of the capillary lumens were measured, and after special computer processing of digital photographs, the total area of the vascular bed in the glomerulus and the area of mesangium in the renal glomerulus were estimated. Results. After four weeks of experiment on rats with diabetes mellitus, some characteristic changes in the morphofunctional state of renal tissues developed: an increase of the area of renal glomeruli, thickening of the basement membrane of glomerular capillaries, an increase in the size of podocytes with a decrease of their number, and foci of nephrosclerosis. Conclusion. Based on the results of the study, characteristic pathomorphological phenomena in the kidneys of rats with experimental diabetes mellitus were recorded, which indicate the development of diabetic nephropathy.

Food products contamination with xenobiotics of various genesis exerts negative influence on population health. According to multiple research performed on various territories in Russia, nitrates are basic contaminants occurring in food products and they make a considerable contribution into higher morbidity with specific nosologic forms, notably pathologies which are primarily caused by factors related to nutrition.The paper focuses on analysis of laboratory research data on nitrates concentrations in food products; the data were collected in Altai region and they are taken from research reports issued by certified test laboratories. The authors also performed their own laboratory research at the Institute for Occupational Hygiene and Industrial Ecology of the RF Public Healthcare Ministry; it allowed to conclude that increased nitrates contents in food products were a vital issue which requires further examination and research performed with high precision laboratory research techniques.We assessed hazard quotients (HQ) for nitrates occurring in vegetables and melons, individual carcinogenic risks (ICR), and population carcinogenic risks (PCR). Our hygienic assessment of nitrates concentrations in food products allowed us to reveal boundaries of risk properties variability.It is necessary to work out recommendations how to reduce exposure of Altai region population to nitrates and to give grounds for priority approaches to administrative decision-making aimed at lowering population risks caused by consumption of nitrates-contaminated food products.

This article presents the results of a study aimed at determining the gastroprotective activity of a number of short-chain peptides against the background of experimental gastropathy in rats. Indomethacin ulcer was used as a model of ulceration. Omeprazole, a drug of basic therapy, was used as a comparison drug. According to the results of the study, two peptides — tetrapeptide LA-4 and hexapeptide LF-6 — demonstrated the gastroprotective activity. The effect was manifested in a signifi cant decrease in the average number of erosions on the surface of the gastric mucosa, their depth, the number of stripe-shaped erosions and Paul’s Index. At the same time, the LF-6 peptide had an effect comparable to that of omeprazole, which makes it promising and interesting for further study.

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