Disinfection plays a significant role in the overall complex of antiepizootic measures. In order to provide manufacturers with a high-quality and cost-effective disinfectant in production conditions and in order to determine the optimal disinfection regime, we conducted production tests of the AKVAdez- NUK 5 disinfectant at the veterinary supervision facility for the stable maintenance of cattle in the Kalacheyevsky district of the Voronezh region. During the production tests, the following concentrations of working solutions were studied: 0.1%; 0.25%; 0,5%. The solution was sprayed with a TORNADOSCO/52 foam generator. The consumption rate was determined as 0.35 l/ m2 with an exposure of 30 min. When using 0.1% concentration of the AQUAdez-NUK 5 working solution with an exposure of 30 minutes, growth was recorded in 16 out of 20 samples during the study for E. coli growth. 4 samples taken from concrete surfaces were disinfected. In samples taken from concrete surfaces to determine the growth of S. aureus, the growth of colonies of microorganisms was observed in all flushes. When using 0.25% AQUAdez-NUC 5 solution in the test, in 16 of the 20 flushes selected to determine the growth of E. coli and 14 out of 20 samples on S. aureus, we did not observe the growth of microorganisms. When treating objects of veterinary supervision with 0.5% AQUAdez-NUC 5 solution after 30 minutes of exposure, there was no growth of microorganisms in 100% of the studied samples on both E. coli and S. aureus during crops from flushes from concrete and wooden surfaces. According to the results of disinfection of the surfaces of the livestock premises with respect to E. coli and S. Aureus, the working solution AQUAdez- NUK 5 showed bactericidal activity in 0.5% concentration with a 30-minute exposure at a consumption of 0.35 l/m2

In the system of veterinary and sanitary measures, disinfection occupies one of the important places, contributing to ensuring the welfare of animal husbandry against infectious diseases, increasing the productivity of animals, poultry and the sanitary quality of products, raw materials and animal feed. The main purpose of disinfection is to break the epizootic chain by influencing its most important link - the factor of transmission of the causative agent of the disease from the source of infection to the susceptible organism. Based on the foregoing, we were assigned the goal of studying the characteristics of the new domestic disinfectant AQUAdez-NUK 5, and to achieve this goal, one of the tasks was to study acute toxicity. In order to study the acute toxicity of AQUAdes-NUK 5 when administered orally, experiments were carried out on male Wister white rats weighing 220-230 g. at doses of 2000, 3000, 4000 and 5500 mg / kg. For the experiments, the rats were divided into groups of 10 animals. 10 animals were used to study each dosage for each disinfectant. One group of ten animals served as a control, which was injected with 0.9% sodium chloride solution. The disinfectant was injected in pure form. After administration, the laboratory animals were monitored for two weeks. The experiment took into account the death of animals and the clinical picture of intoxication. In the course of laboratory studies, we determined that AQUAdez-NUK 5 belongs to the III hazard class in accordance with GOST 12.1.007-76 (moderately hazardous compounds), as well as the presence of a weak irritant effect, and therefore we took appropriate safety measures during work with this disinfectant.

Currently, there is a problem in satisfying livestock enterprises with cheap, highly effective disinfectants. Import substitution of the vacated market segment requires optimization of the increasing costs of livestock companies. In this regard, preclinical studies of the AKVAdez-NUK 5 disinfectant were developed and studied, in particular, indicators of acute toxicity. In the experiment, males of the Wistar breed weighing 220-230 g were used, to whom the test solution was injected intragastrically, using a pin-shaped needle, in doses of 2000, 3000, 4000 and 5500 mg/kg. Using of analytical and statistical tools in data processing allowed us to state that for the disinfectant AQUAdez-NUK 5 has followed levels for lethal doses. LD16=2864,91mg/kg; LD50=3676,22±347,37mg/kg; standard error for LD50 is 347,37 mg/kg; LD84=4585,86 mg/kg; LD99=5040.69 mg/kg. The clinical picture of acute poisoning in rats was manifested by short-term excitement, followed by depression. Tachypnea, nasal discharge, impaired coordination of movement and clonic-tonic seizures were diagnosed in laboratory animals. The rats huddled in groups. The death of animals occurred due to respiratory arrest. By analyzing the results of an acute experiment and recording the frequency of animal deaths by observation days, the average time of animal death was calculated, which was 55.92 hours. Taking into consideration of the results, we can conclude increasing in the administered dose of the drug accelerates the onset of death of a laboratory animal. Up to the level of 4000 mg / kg, only one experimental rat died on the third day after the introduction of 3000 mg / kg. With the introduction of large dosages, lethality increased sharply, reaching 100% with the introduction of 5500 mg of AQUAdez‑NUC 5 per 1 kg of weight. In accordance with the classification of AQUAdez‑NUC 5, it can be attributed to drugs of an acute type of action with a moderate ability to cumulate. According to government standard 12.1.007-76, AQUAdez-NUK 5 belongs to hazard class III (moderately hazardous compounds).

The article provides information on the determination of the bacteriostatic and bactericidal activity of the disinfectants AQUAdes-NUK 5, Desinfexan and IncimaxxDES in relation to Salmonella typhimurium. The activity of disinfectants AQUAdes-NUK 5, Desinfexan and IncimaxxDES in the framework of determining their bactericidal activity and the severity of bacteriostatic properties was studied by the method of serial dilution, in which daily cultures of Salmonella typhimurium were used. During the research, the minimum concentrations were established at which bactericidal activity was manifested and a pronounced bacteriostatic effect was obtained. After processing the results obtained, we can conclude that the disinfectant AQUAdez-NUK 5 has a bacteriostatic effect on S. typhimurium at a concentration of 0.004%, but a pronounced bactericidal effect occurs only at a concentration of 0.008%. The degree of effect of IncimaxxDES disinfectant on S. typhimurium characterizes this agent as highly effective for disinfection when contaminated with this pathogenic microorganism. In particular, the bacteriostatic effect could be ascertained when the concentration reached 0.002%, the bactericidal effect at the 0.008% concentration, which indicates the high efficiency of the drug.Disinfexan has a pronounced antimicrobial effect, the bactericidal effect on S. typhimurium occurred at a similar dilution of 0.002%, and the bacteriostatic effect was manifested at lower dilutions of 0.0005%. In studies on the setting of control during bacteriological examination of the means AQUAdes-NUK 5, IncimaxxDES and Desinfexan, the growth of microorganisms was observed in test tubes into which the culture of S. typhimurium was enucleated. There was no growth in a test tube with the addition of distilled water to the BCH. The results obtained prove that the new disinfectant AQUAdes-NUK 5 exhibits pronounced bactericidal activity against the test microorganism S. typhimurium.