A new digital holographic microscopy scheme for multi-spectral quantitative phase imaging is proposed and implemented. It is based on acousto-optic filtration of wide-band low-coherence light at the entrance of a Mach-Zehnder interferometer, recording and digital processing of interferograms. The key requirements for the acousto-optic filter are discussed. The effectiveness of the technique is demonstrated by calculating the phase maps of human red blood cells at multiple wavelengths in the range 770–810 nm. The scheme can be used for the measurement of dispersion of thin films and biological samples.

We report on wavelength-multiplexed digital holographic imaging based on simultaneous Bragg diffraction of wideband light by several ultrasound waves of different frequencies in crystalline media. This technique is easy to implement, avoids spectral scanning, and is applicable in various digital holography schemes. It also enables single-shot acquisition of a few spectral fringe patterns by a single monochrome sensor and wavelength demultiplexing of the resulting interferogram. We have assembled a Mach–Zehnder interferometer with an acousto-optical tunable filter operating in the multifrequency mode and have validated the proposed technique by dual-, three-, and four-wavelength quantitative phase imaging of the test samples.

No abstract

No abstract