The study investigated the susceptibility pattern of gram negative bacteria isolated from pregnant women attending antenatal at Bishop Murray hospital, Makurdi Benue State Nigeria. A total of 40 clean catch midstream urine samples were collected from the hospital laboratory in sterile bottles and taken to the school laboratory. Inoculation of the samples to the media was done using the streak method. Twenty eight (28) samples contained gram negative isolates identified by colony morphology, microscopy and biochemical test which included: Escherichia coli 12(30%), Pseudomonas aeruginosa 9(22.2%), and Klebsiella pneumoniae 7(17.5%).The susceptibility of the isolated bacterial strains were tested against 10 different antibiotics using the disc diffusion method and their activity was evaluated by observing zones of inhibition, measured and recorded. The results showed that E. coli, Pseudomonas aeruginosa and Klebsiella pneumonia were highly susceptible to ciprofloxacin (with zone inhibition 26.50 mm, 24.50 mm, and 25.00 mm respectively), Augmentin (21.3 %) Tarivid (20.6 %), Streptomycin (14.3 %), Gentamycin(18.0 %), Perfloxacin (10.0 % with Pseudomonas completely resistant), Septrin(15.3 %), Amoxacillin (12.9 % with E. coli completely resistant) Sparfloxacin (11.0 % with Klebsiella resistant). The isolates were resistant to chloramphenicol with no zone of inhibition