Citronella oil is one of the non-timber forest products (NTFP) and commercially obtained from the distillation of the leaves of the Cymbopogon nardus L. This study aims to determine the effect of the size of the distillation raw material and storage time of the raw material on yield and determine the antimicrobial properties of citronella oil from plants in the production forest Register 40 KPH Gedong Wani, Lampung Selatan Regency. The research was conducted using a complete randomized block design (RAKL) with two factors, namely the size of the distillation raw material (whole leaf, 5 cm, 10 cm, and 15 cm) and the storage time of the raw material (fresh, 2 days, and 4 days) which was carried out in 3 replications. The variables measured were yield, specific gravity, and solubility of citronella oil in alcohol. The inhibition ability test of citronella oil against bacteria using essential oil obtained from distillation at the highest yield conditions, namely fresh leaves and leaf size of 5 cm using the diffusion well method. The results showed that the storage time factor, the size of the distillation raw material, and the interaction between the storage time and the size of the raw material had a significant effect on the yield of citronella oil. The highest yield of citronella oil was achieved in the condition of fresh leaves with a size of 5 cm, namely 2,09%. Density and solubility in alcohol of citronella oil produced met SNI 06-3953-1995 standard, respectively 0,8718-0,8928 g/ ml and 1:2. Citronella oil has strong antibacterial properties against Propioni acne, Escherichia coli, Staphylococcus aureus, and Bacillus cereus with inhibition zone diameters were 40,20 mm, 18,36 mm, 13,07 mm, and 18,80 mm, respectively. Citronella oil from plants in Register 40 KPH Gedong Wani has potential as a raw material for the cosmetic and disinfectant industry. Keywords: antimicrobial, citronella oil, yield

This study aims to isolate the glucoamylase enzyme from Aspergillus niger to produce liquid sugar from tapioca flour. Enzymes are produced in a batch system laboratory-scale bioreactor. The fermentation substrate in an agar medium modified with the addition of starch. Fermentation was carried out for 168 hours at room temperature with an agitation speed of 120 rpm. The manufacture of liquid sugar is done by adding an enzyme as much as 0.3; 0.4; and 0.5 ml while the substrate concentration was varied by 20%; 30%; and 40%. The results showed that A. niger produce extracellular enzymes such as amylase and glucoamylase, particulary at exponential phase and the initial stationary phase. Enzymatic hydrolysis of starch consists of two stages, namely liquification and saccharification. In the liquification process, the enzyme α-amylase is used to break down starch containing amylose and amylopectin into dextrins. Next in the second stage is the saccharification process, where dextrin is hydrolyzed into glucose with the help of the glucoamylase enzyme. Optimal liquid sugar production can be done by adding 0.5 mL of crude enzyme extract at 30% (w/v) tapioca concentration. Further research is recommended to carry out enzyme purification to produce liquid sugar products with higher sugar purity.