А. В. КАРЕЛОВ, кандидат біологічних наук, старший науковий співробітник Інститут захисту рослин НААН, ДУ «Інститут харчової біотехнології та геноміки НАН України» І. О. СОЗІНОВ, старший науковий співробітник Інститут захисту рослин НААН, Н. О. КОЗУБ, кандидат біологічних наук Інститут захисту рослин НААН, ДУ «Інститут харчової біотехнології та геноміки НАН України»

The results of the investigations of resistance winter common wheat varieties to leaf rust are given. The high resistance to the pathogen manifest varieties that contain resistance genes: Lr9, Lr19, Lr37, Lr42 + Lr24, Lr43 (Lr21 + Lr39) + Lr24, Lr9 + Lr26, Lr10 + Lr24 are ascertained. The genes Lr13, Lr34, Lr37 in combination with other resistance genes provides long-term protection to leaf rust wheat. Winter wheat varieties, created at the V. M. Remeslo Myronivka Institute of Wheat, contain resistance genes Lr23, Lr24, Lr26, Lr34. The varieties Vesta, Snizhana, Demetra are protected by the resistance genes Lr26 + Lr34, variety Zolotokolosa– Lr24 + Lr34, Ekonomka – Lr3 + Lr26, Myronivska storichna – Lr3 + Lr23 + Lr10 + Lr26. The allele Lr34 (+) is contained in varieties: Kryzhynka, Vesta, Snizhana, Volodarka, Demetra, Vdiachna, Pamiati Remesla, Sviatkova, Podolianka, Berehynia myronivska, MIP Dniprianka, and Balada myronivska.  Sustainability is an important element of an integrated system of plant protection against many diseases, and to ensure increased yields it is necessary to create and distribute sustainable varieties that will be an environmentally promising way to develop the agro-industrial complex of Ukraine.

Purpose. Creation of new breeding material of soft winter wheat, highly resistant to diseases of the ear and pest colonization for use in the breeding process. Methods. The studies were carried out in 2017–2020 under conditions of artificial inoculation of wheat plants with pathogens of common bunt and fusariosis of the ear in field infectious nurseries of the Department of Plant Protection of the V. M. Remeslo Institute of Wheat of NAAS. An artificial infectious background of common bunt was created according to the method of A. I. Borggard-Anpilogov, which consists in contamination of seed material with spores several days before sowing. An artificial infectious background of fusarium ear blight was created by spraying soft winter wheat plants in the flowering phase with a suspension of spores isolated from the local pathogen population. Results. According to the results of the conducted research, highly resistant (up to 5% ear dama­ge) combinations of hybrids of the fourth generation of soft wheat were selected against the causative agent of fusarium: ‘Berehynia Myronivska’ / ‘Nobeoka bozu’ had a thrips population of 5.2 ind./ear, and cereal leaf beetle – 35,0 ind./m2 and ‘Horly­tsia myronivska’ / ‘C-Lokia’, the thrips population of which was 5.0 ind./ear, cereal leaf beetle – 2.0 ind./m2. On an artificial infectious background of fourth-generation hybrids, in terms of resistance to common bunt, the crossing combinations ‘Berehynia Myronivska’ / ‘Horianka’, ‘Lehenda Myronivska’ / ‘Nana’ were selected, which were affected by common bunt from 15 to 20%, and thrips population was 2.8–8.6 ind./ear, cereal leaf beetle – 5.0–6.0 ind./m2. The highest indicators of the length of the ear, the number of grains in the ear and the mass of grain from the ear were obtained in the combinations of ‘Oberih Myronivskyi’ / ‘Maris Templer’ and ‘Berehynia Myronivska’ / ‘Horianka’, which were created in accordance with the breeding programs of soft winter wheat for resistance against fusarium head blight and common bunt. Conclusions. The constant lines of soft winter wheat, isolated by complex resistance against diseases and pests, are used in the breeding process of the V. M. Remeslo Institute of Wheat of NAAS and the National Center of Plant Genetic Resources of Ukraine (The Plant Production Institute named after V. Ya. Yuriev, Kharkiv).

In today's environment, an important task of agricultural production is to increase yields and improve the quality of agricultural products. A reliable and environmentally beneficial factor for sustainable yield increase and improvement of grain quality is the development of new varieties with group resistance to diseases and a high level of adaptability. The effectiveness of selection for immunity directly depends on the diversity of donor resistance genes, and the search for new sources of resistance has been and remains an urgent issue. The aim of the study was to determine the level of infection of soft winter wheat varieties with Fusarium head blight pathogens and to identify them. Varieties of soft winter wheat from different breeding institutions of Ukraine were used in the research: Institute of Plant Industry named after V.Y. Yuriev NAAS (IPI), Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine (IPPG), Institute of Plant Protection NAAS (IPP), Institute of Irrigated Agriculture NAAS (IA), National Research Center "Institute of Agriculture NAAS" (NRC IA). Phytopathological analysis was used to determine the species composition of the Fusarium pathogen, and the proportion of species was determined by the ratio of infected wheat grain samples to their total number. A higher (15-18%) level of Fusarium infection intensity was observed in the varieties Perfect, Turunchuk, Ovid, Vodograi, and Mirolyubna. During the years of research, winter wheat grain was colonized by the following species of Fusarium ear blight: F. sporotrichiella, F. monilifopme, F. graminearum, F. culmorum, F. avenaceum, F. gibbosum F. sambucinum and F. oxysporium. The species F. monilifopme and F. oxysporium dominated, with a prevalence of 16.2 and 1.7% on wheat grain, respectively. The winter wheat varieties with the lowest (1.2-4.5%) damage by pathogens of the genus Fusarium are recommended for use in wheat breeding for immunity. The use of stable source material in practical breeding will help to curb the growth of pathogen infection without the use of chemical protection agents.

Diseases of field crops substantially reduce the yield and quality of the obtained agricultural products. Underselection of gross grain harvest due to diseases and pests can reach about 25-30% annually. This necessitates the creation of resistant varieties for winter wheat selection. The purpose of this study was to create a new highly productive selection material with complex resistance against the main most harmful diseases for further use in the selection of winter wheat. In the phase of emergence of plants in the tube, winter wheat plants were infected with spores of the causative agent of brown rust according to E.E. Heschele’s method. To create an artificial infectious background of brown rust, a synthetic population of the pathogen (Institute of Plant Protection of the National Academy of Agrarian Sciences) was used as a reservoir of infection – a susceptible variety of Myronivska 10. To create an artificial infectious background of powdery mildew, a local population and an infectious reservoir of the American variety Keprock were used. In the F3 hybrid nursery against the provoking background of powdery mildew, combinations highly resistant to the pathogen (up to 5%) were selected, created with sources of resistance: Zdar, Fakon, Pi170911, Bongo – Svitanok Myronivskyi/Zdar, Kolos Myronivshchyny/Fakon, Berehynia Myronivska/Pi170911, Dostatok/Bongo. Resistance against the pathogen (up to 10.0%) was observed in two crossing combinations (Gorlytsia Myronivska/Gloria and Remeslivna/Wervok). Crossing combinations created with sources of resistance were selected on the artificial infectious background of hybrids of the fourth generation for resistance to brown rust: Flex, V 1275, Tobarzo, 203-238. It is worth noting the hybrids: Oberih Myronivskyi/Flex, Smuhlianka/V 1275, Monotyp/Tobarzo, Kolos Myronivshchyna/203-238. The largest number of grains in an ear was obtained from the combination Svitanok Myronivskyi/Zdar (61.5 pieces), and the largest weight of grain from an ear was obtained from the combinations Horlytsia Myronivska/Gloria and Berehynia Myronivska/Pi170911 (2.38 g and 2.37 g, respectively). The Oberih Myronivskyi/Flex combination stood out for its resistance to brown rust and performance elements. The samples selected based on the results of the study are used as valuable raw material to create new winter wheat varieties resistant to diseases in the Forest Steppe of Ukraine